Use of Objective Lenses and Reflectors in General Optical Microscopes

Jan 04, 2024

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Use of Objective Lenses and Reflectors in General Optical Microscopes

 

Use of low magnification objective lens
①Turn the coarse focusing screw by hand, make the lens barrel descend slowly, and at the same time, gaze at the objective lens from the side with both eyes, and stop when the objective lens and the slide of the carrier table are 2~3mm apart.


②Gaze into the eyepiece with the left eye (note that the right eye should be open at the same time), and turn the coarse focusing spiral so that the barrel of the mirror rises slowly until the object is clear. If it is not clear, adjust the fine focusing spiral until it is clear.


Use of the high-magnification objective lens
Before using the high-magnification objective lens, you must first use the low-magnification objective lens to find the object of observation, and adjusted to the centre of the field of view, and then turn the converter and then change the high-magnification lens. The brightness of the field of view is dimmed by changing to a high magnification lens, so a larger aperture and the concave side of the reflector are usually used, and the fine focusing screw is adjusted. The number of objects viewed becomes smaller, but the size becomes larger.


Use of Reflectors
Reflectors are usually used in conjunction with a diaphragm (or aperture) to adjust the brightness in the field of view. Reflectors come in flat and concave surfaces. When focusing, if the light in the field of view is too strong, use the flat side of the reflector, and if the light is still too strong, use a smaller aperture at the same time; conversely, if the light in the field of view is weak, use a larger aperture or use the concave side of the reflector.

3 Digital Magnifier -

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