Use of ordinary optical microscope objectives and reflectors
Use of low magnification objectives
① Turn the coarse focus screw by hand to slowly lower the lens barrel. At the same time, look at the objective lens from the side. Stop when the distance between the objective lens and the glass slide on the stage is 2 to 3 mm.
② Use your left eye to look into the eyepiece (note that your right eye should be open at the same time), and turn the coarse focus screw to slowly raise the lens barrel until you can see the object clearly. If it is not clear, adjust the fine focus screw until it is clear.
Use of high magnification objective lenses
Before using a high-magnification objective lens, you must first find the object to be observed with a low-magnification objective lens and adjust it to the center of the field of view, then turn the converter and change to a high-magnification lens. After switching to a high-magnification lens, the brightness in the field of view becomes darker, so generally choose a larger aperture and use the concave surface of the reflector, and then adjust the fine focus screw. The number of objects viewed becomes smaller, but their size increases.
Use of reflectors
Reflectors are often used in conjunction with a shutter (or aperture) to adjust the brightness within the field of view. Reflectors have flat and concave surfaces. When facing the light, if the light in the field of view is too strong, use the flat surface of the reflector. If the light is still too strong, use a smaller aperture at the same time; conversely, if the light in the field of view is weak, use a larger aperture or use a reflector. concave surface.
Precautions:
a. The microscope is a precision instrument. It should be operated gently and it is not allowed to be disassembled casually. If there is a fault, it should be reported to the instructor in time for processing. Do not interchange eyepieces or objective lenses between different microscopes.
b. When observing the temporary slide, do not let the water in the slide flow to the stage, let alone allow acids, alkalis and other chemicals to come into contact with the microscope.
c. When you find that the objective lens or eyepiece is not clean, wipe it in a straight line with lens cleaning paper. Do not wipe with fingers, handkerchiefs, cotton cloth, etc. to avoid scratching or contaminating the lens. If there is oil on the lens, you can first wipe it with lens cleaning paper dipped in a little xylene, and then wipe it with clean lens cleaning paper.
