What are the common problems of using a light microscope
Optical microscope is a high-precision optical instruments, a price of 1,000, more expensive hundreds of thousands of a, for the enterprise, this is also a great expense. In order to enhance the application of optical microscope quality, increase the use of the period, grasp the application of optical microscope common problems are obliged to. Naturally, people do not need to be overly nervous, if you grasp these aspects, you can easily deal with the application of optical microscopes in the whole process of the problem.
1, people in the application of optical microscope, first pay attention to the overall installation of the microscope is not loose, for example, optical microscope has been applied for a long time, will cause the support bracket is loose, the objective lens is installed too loose and other problems. That too loose will jeopardize the actual effect of the test, but also very easy to destroy the optical microscope.
2, the use of focusing screw common problems. People in the whole process of operation is very easy to focus immediately in the high magnification, or no matter the barrel of the lens is raised or lowered, both eyes have always been in the eyepiece to see the line of sight, or is not to grasp the critical point of the object distance, the object distance is adjusted to the 2-3 centimeters but also to the rise, and rotate the focusing spiral rate is rapid. This kind of problem is very easy to cause into the objective lens camera lens reaction to feel mounted, damage to the mount or camera lens. For the above practical problems, the net editor would like to say, the guys in adjusting the lens focus must be in the low magnification down, first rotate the coarse focusing helix, so that the barrel of the lens is gradually lowered, the objective lens is close to the coverslip, but pay attention to do not have to let the objective lens to meet the coverslip, in the whole process of the two eyes to look at the objective lens from the side, followed by the right eye towards the eyepiece to gaze inside, and gradually in the opposite direction, adjusting the coarse focusing helix, so that the barrel of the lens rises, until the false image is seen. Until you see the false image, in addition to the students that the general optical microscope object distance of 1 centimeter above and below, so if the object distance is far more than 1 centimeter, but did not see the false image, it may be specimen collection is not in the line of sight or rotate the coarse focusing spiral rate is too fast, then should be adjusted to the mounting site, and then repeat the process, when there is a blurred image in the line of sight, you need to switch to a fine focusing spiral adjustment, only then can be smaller to find the false image, and then the right eye toward the eyepiece. Only in this way can the scope of searching become smaller and the rate of searching for false images be improved.
Application of optical microscope common problems:
3, optical microscope to the light of the problem. To the light is a very important step in the application of optical microscope, remember, must use low magnification on the light, when the light source is strong with a small focal length, plane mirror, and the light source is poor when the use of large aperture lenses, concave mirrors, rotating the rearview mirror can not be used with one hand will be the mirror wrenched out to rotate with two hands, when the see a uniformly bright ring of vision before doing so. Never move the optical microscope at will after the light is right, in case the light source can not accurately according to the rearview mirror into the hole of the pass line.
4, the difficulty of changing the objective lens. After the application of low magnification mirror to change to high magnification mirror, people usually like to use their fingers to immediately push the objective lens, think that is more effort, but that is very easy to make the optical axis of the objective lens is tilted, the reason is that the raw material of the converter is too soft, high precision, the external threads are not proportional to the support is very easy to loosen. Once the outer thread is destroyed, all the converter will be reported. Therefore, the net editor proposes that people hold the converter of the next layer of the rotating plate to change the objective lens.
5, high-magnification microscope with the eye of the problem. Guys with high magnification microscope object block, the eyes should be in addition to open, the right eye to the eyepiece gaze, that is, the right eye should be as close as possible to the eyepiece, the right eye try to the line of sight to gaze, do not have to force to cover the right eye or decisively close the right eye, it is inconsistent with the provisions of the test of the observation and investigation, and often that the right eye is easy to fatigue, and in addition to ensure that the observation and investigation of the side of the plotting.
