What are the common problems when using optical microscopes?
An optical microscope is a high-precision optical instrument. The price of one unit starts at 1,000, and the more expensive one is hundreds of thousands. For enterprises, this is also a huge expense. In order to improve the application quality of optical microscopes and extend their service life, it is imperative to understand the common problems encountered in the application of optical microscopes. Of course, you don’t need to be too nervous. As long as you master these aspects, you will be able to easily solve the problems in the microscope use process.
1. When using an optical microscope, first pay attention to whether the overall installation of the microscope is loose. For example, if the optical microscope is used for a long time, problems such as loose support brackets and too loose installation of the objective lens may occur. If it is too loose, it will affect the inspection effect and easily damage the microscope.
2. Frequently asked questions about using quasi-focus spirals. It is easy for everyone to focus directly under high magnification during operation, or whether the lens tube is raised or lowered, the eyes are always looking into the eyepiece, or whether the critical point of the object distance is grasped, the object distance is adjusted to It will still rise when it reaches 2 to 3 centimeters, and the speed of rotating the accurate focus spiral is very fast. These problems can easily cause the objective lens to react to the loading film, damaging the loading film or lens. Regarding the above operational issues, the editor would like to say that when adjusting the focal length of the lens, you must lower the low-magnification lens and first turn the coarse focus screw to slowly lower the lens barrel and bring the objective lens close to the cover glass, but be careful not to Let the objective lens meet the cover glass. During this process, both eyes should look at the objective lens from the side. Then gaze into the eyepiece with your right eye, and slowly adjust the coarse focus screw in the opposite direction, so that the lens barrel slowly rises until you see A virtual image will do. In addition, explain to students that the object distance of a general optical microscope is about 1 cm. Therefore, if the object distance is far more than 1 cm but no virtual image is seen, it may be that the specimen was not collected within the line of sight or the rotation was inaccurate. The focus spiral speed is too fast. At this time, the film loading position should be adjusted, and then the above process is repeated. When a blurry virtual image appears in the line of sight, it is necessary to switch to the fine focus spiral adjustment. Only in this way can the focus be reduced. Search range and increase the speed of finding virtual images.
Frequently Asked Questions about Application of Optical Microscopy:
3. The problem of light in optical microscopes. Correcting light is a very important step when applying an optical microscope. Remember, you must use a low-power lens to illuminate the light. When the light source is strong, use a small focal length and flat mirror. When the light source is poor, use a large aperture lens and concave mirror. Rotate the light. When using the rearview mirror, you cannot pull it out with one hand. You must use both hands to rotate it until you see a uniform and bright circular sight. After the light is aligned, be sure not to move the microscope to avoid the light source not accurately entering the light-passing hole through the rearview mirror.
4. The problem of objective lens transformation. After using a low-magnification lens, switch to a high-magnification lens. People usually like to use their fingers to push the objective lens directly. They think this is more labor-saving, but this can easily cause the optical axis of the objective lens to tilt. The reason is that the material of the converter is too soft and delicate. The degree is high and the external thread support is uneven and easy to loosen. Once the external thread is damaged, the entire converter will be billed. Therefore, the editor recommends that everyone hold the lower rotating plate of the converter to change the objective lens.
5. Problems with using eyes for high-power microscopes. When using a high-power microscope object, you should open your eyes at the same time and gaze into the eyepiece with your right eye. That is, the right eye should be as close as possible to the eyepiece. Try to gaze into the line of sight with your right eye. Do not cover your right eye hard or close your right eye decisively. Eyes, this does not meet the observation requirements of the test. Often, the right eye is prone to fatigue. At the same time, it is not possible to observe and draw at the same time.
