What are the differences between using oil-filled lenses and ordinary lenses?
When using the oil mirror, a drop of tar needs to be dropped on the glass slide, and the objective lens cannot detach from the oil drop during the upward process. Moreover, the magnification of the oil mirror is larger than that of a regular objective lens.
When using oil mirrors, attention should be paid to:
1. After using the oil lens, first wipe off the cedar oil on the lens and specimen with a small amount of xylene on the lens wiping paper, and then wipe it clean with dry lens wiping paper. After adding tar, the field of view becomes noticeably darker. It is necessary to raise the collector to the highest position and turn the aperture to the maximum. Before using an oil mirror, it is necessary to first place the target observed through a low magnification lens and a high magnification lens in the center of the field of view.
2. Xiangbai oil is a specialized oil for oil mirrors. Dropping a liquid with a refractive index of 1.5 under a 100x oil mirror can significantly increase the resolution of the mirror and improve the observation effect of the microscope. Xiangbai oil has a refractive index of 1.52, making it an excellent oil for microscope oil mirrors.
3. When using a microscope oil mirror, the microscope must be upright on the table, and the arm of the mirror must not be bent to tilt the stage, in order to avoid the overflow of tar, affecting observation, and contaminating the table.
Why use cedar oil when observing microorganisms with an oil mirror
Because the magnification of the oil mirror is higher, while the lens is very small. When light passes through media objects of different densities, some of the light will refract and dissipate, resulting in less light entering the mirror tube and a darker field of view, making it difficult to observe the object clearly. Dropping cedar oil with a refractive index similar to that of glass between the lens and the glass slide increases the amount of light entering the oil mirror, enhances the brightness of the field of view, clarifies the object image, and facilitates observation. Therefore, cedar oil should be used when observing microorganisms through the oil mirror.
