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What are the factors that affect the resolution of a microscope?

Jan 18, 2025

What are the factors that affect the resolution of a microscope?

 

1. The microscope is cloudy and unclear


Main reason: Unclean or moldy lenses.
Exclusion method: When there is dust or dirt on the lens, use a brush or feather to remove it, then use lens paper or a degreasing cotton dipped in a small amount of anhydrous alcohol or ether to carefully wipe along a circular trajectory, but do not let the wiping liquid flow away.


2. The edge of the indentation cannot be clearly seen inside the mirror
Main reason: Some lenses are loose.


Exclusion method: Re tighten the loose parts of the lens.


3. The scale value of the reading microscope does not coincide with the scale of the standard ruler


Main reasons: Loose objective lens or missing washer at the connection between objective lens and barrel, resulting in changes in focal length.


Exclusion method: Tighten the objective lens. If the washer is missing, its thickness should be repeatedly adjusted and a suitable washer should be installed until the position with the smallest scale error.


4. The scale value of the reading microscope is larger than that of the standard ruler


Main reason: The tube has grown, possibly due to loose tube joints.


Exclusion method: Re tighten the tube joint.


Maintenance of microscope
1. Regular maintenance
(1) If the indoor environment is damp, optical lenses are prone to mold and fog. Once the lens becomes moldy, it is difficult to remove. The lenses inside the microscope are more harmful to moisture due to the inconvenience of wiping. Mechanical parts are prone to rusting when exposed to moisture. In order to prevent moisture, when storing microscopes, in addition to choosing a dry room, the storage location should also be away from walls, floors, and sources of moisture. 1-2 bags of silicone gel should be placed inside the microscope box as a desiccant. And often bake the silicone. After its color turns pink, it should be baked in a timely manner before continuing to use.


(2) Dust falling onto the surface of dust-proof optical components not only affects the passage of light, but also generates large stains after amplification by the optical system, which affects observation. Dust and sand particles falling into the mechanical parts can also increase wear and tear, causing movement obstruction and posing significant hazards. Therefore, it is necessary to regularly maintain the cleanliness of the microscope.


(3) Anti corrosion microscopes cannot be placed together with corrosive chemical reagents. Such as sulfuric acid, hydrochloric acid, strong bases, etc.


(4) The main purpose of heat prevention is to avoid the opening and detachment of lenses caused by thermal expansion and contraction.

 

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