What are the methods to improve the resolution of the microscope
The resolution of a microscope refers to the ability of the smallest distance between two object points that can be clearly distinguished by the microscope.
Its calculation formula is σ=λ/NA
In the formula, σ is the resolution; λ is the wavelength of the light; NA is the numerical aperture of the objective lens (NA=nsina/2, n is the refractive index of the medium, a is the aperture angle, that is, the aperture angle of the sample to the objective lens). The resolution of the visible objective lens is determined by two factors: the NA value of the objective lens and the wavelength of the illumination source. The larger the NA value, the shorter the wavelength of the illumination light, and the smaller the σ value, the higher the resolution.
To increase the resolution, i.e. reduce the value of σ, the following measures can be taken
(1) Reduce the wavelength λ value and use a short-wavelength light source.
(2) Increase the medium n value to increase the NA value (NA=nsina/2).
(3) Increase the value of aperture angle a to increase the NA value.
(4) Increase the contrast between light and dark.
