What is the classification of microscope filters?
1. The field of view of the daylight filter will turn yellow when artificial light is used. The details of red staining and yellow staining in stained specimens are not easy to distinguish. The artificial light source can be changed to approximate natural light by using light blue No. 209 filter. The light blue slides attached to ordinary microscopes belong to this type of filter.
2. Green filter When observing the red details on the specimen, the use of the green filter can increase the contrast of the details of the specimen, that is, the contrast. Even more important when performing photomicrography on black and white negatives. Because general panchromatic film is particularly sensitive to blue and green light, but slow to red light. Hematoxylin-eosin stained specimens are too bright without a green filter. When using a green filter, the blue color of the specimen details is absorbed and a natural object image appears. Therefore, when performing photomicrographs on black and white negatives, the No. 211 transmission curve 510-590nm filter from the Livi filter series is the best.
3. Yellow-green filter The yellow-green filter with high absorption rate is very important for the observation of unstained specimens with low contrast such as cultured cells.
4. The 510nm No. 212 filter in the Livi series of yellow filters is also very good for the observation of living cells. The contrast in the microscopic R image is too large, and when the details of the object image are not clear, the blue details of the specimen can be properly displayed with a yellow filter.
5. The 209th filter in the Livi series of blue filters is transparent at 430-480nm, and is best used for observing yellow and green dyed specimens and their photomicrographs.
6. Heat-absorbing filter In the early days, a liquid heat-absorbing filter device was used to prevent infrared heat radiation from damaging the optical unit. The illuminating light source set of a modern large microscope is equipped with a heat-absorbing glass filter that can be placed into the light path or placed out.
