What is the depth of field of a biological microscope
Another name for the depth of focus of a biological microscope is scenery, and depth of focus is short for depth of focus. How to understand the depth of focus? When we observe a three-dimensional object, we slowly adjust the coarse and fine adjustments of the biological microscope so that the objective lens is close to the object under inspection. At this time, the part of the top of the object on the plane closest to the objective lens is the clearest, not only the part on this level is clear, but also the part with a certain thickness below is also clearly recognizable. This clearly resolvable thickness is the depth of field of the microscope. The greater the depth of field, the more objects can be seen vertically. Otherwise, only a thin layer can be seen clearly.
Depth of field is related to the technical parameters of the microscope. First, the depth of focus is inversely proportional to the total magnification and the numerical aperture of the objective lens. Other parameters remain unchanged. The larger the magnification, the smaller the depth of focus of the microscope. Similarly, the smaller the numerical aperture of the other parameters. The larger, the smaller the depth of focus. Secondly, the depth of focus has a certain relationship with the resolution. The larger the depth of focus, the lower the resolution of the biological microscope.
In order to solve the above several contradictions and want to obtain the image effect of high depth of field, high magnification and high resolution, we can solve it through digital imaging systems. Some digital imaging systems have the function of layer merging. When we want high depth of field and high For magnification and high-resolution images, the images of high magnification and low depth of field of the entire object at all levels are transmitted to the computer through the CCD, and then the image processing software with layer merging function matched with the CCD is used to convert each After merging the high-definition images of different layers, we can finally get an image with high depth of field, high magnification and high resolution.
