What is the difference between fluorescence microscopy and laser confocal microscopy?
Different principles
1, fluorescence microscope: ultraviolet light as a light source, used to irradiate the object to be examined, so that it emits fluorescence, and then observe the shape of the object and its location under the microscope.
2, laser confocal microscope: in the fluorescence microscope image on the basis of the addition of laser scanning device, the use of ultraviolet light or visible light to stimulate the fluorescent probe.
Different characteristics
1, fluorescence microscope: used to study the absorption of substances in the cell, transport, distribution and localisation of chemical substances. Some substances in the cell, such as chlorophyll, can fluoresce after irradiation by ultraviolet light; some other substances itself can not fluoresce, but if stained with fluorescent dyes or fluorescent antibodies, fluorescence can also fluoresce by ultraviolet light irradiation.
2,Laser confocal microscope: Use computer for image processing, so as to get the fluorescence image of the internal microstructure of cells or tissues, as well as in the subcellular level to observe such as Ca2+, pH, membrane potential and other physiological signals and changes in cell morphology.
Different uses
1, fluorescence microscope: fluorescence microscope is the basic tool for immunofluorescence cytochemistry. It is composed of light source, filter plate system and optical system and other major components. It is the use of a certain wavelength of light to stimulate the specimen to emit fluorescence, through the objective lens and eyepiece system to magnify the specimen to observe the fluorescence image.
2,Laser confocal microscope: laser scanning confocal microscopy has been used for cell morphology positioning, three-dimensional structure reorganisation, dynamic change process research, and provide quantitative fluorescence determination, quantitative image analysis and other practical means of research, combined with other related biotechnology, in the field of molecular and cellular biology, such as morphology, physiology, immunology, genetics and other fields have been widely used.
