What is the laser confocal microscope's resolution?
True color confocal microscope and laser scanning confocal microscope, the two are basically the same in imaging principle, the biggest difference is that the illumination source is different. 1. Laser scanning confocal microscope The illumination source of laser scanning confocal microscope is laser, That is, monochromatic light. The actual imaging process is based on the intensity of the reflected light of the monochromatic laser from the observed object. Because it is monochromatic light illumination, the color cannot be distinguished. For the same sample in the same field of view , the colors are different, but different tissues or components with the same light intensity reflected by the monochromatic laser cannot be distinguished. It is easy to produce the phenomenon of the same phase and different colors, and the same color and different phases, which is not conducive to the correct identification of microstructures and components. 2. True color coherence The light source of the focal microscope true color confocal microscope is a xenon light source, that is, white light. The actual imaging process is to comprehensively image the shape (including color) of the object under the condition of white light illumination. Due to the polychromatic light illumination and imaging, the real The color confocal microscope can reflect the color and shape of the object more realistically, avoiding the phenomenon of the same phase and different colors and the same color and different phases produced by the laser scanning confocal microscope. The observer can distinguish and judge the composition and composition of the sample through the color. Organization. In this respect, its resolution is much stronger than the comprehensive analysis of the laser scanning confocal microscope: under the observation conditions of samples with color differences, the true color confocal microscope avoids the same phase and different colors produced by the laser scanning confocal microscope, The phenomenon of the same color and different phases occurs, and the observer can distinguish and judge the composition and organization of the sample through the color. Under this condition, the resolution of the true color confocal microscope is higher than that of the laser scanning confocal microscope. In the monochromatic sample Under certain observation conditions, the resolution is close to the respective technical indicators. However, in the actual observed samples, most of the different tissues and components have color differences. Corresponding to the samples with no or small color differences Similarly, artificial staining (such as corrosion treatment) can be used to improve the resolution of the image. In this regard, the laser scanning confocal microscope is powerless. In addition, the resolution is a technical indicator that can be achieved under specific conditions , when the technical conditions are not met in actual use (in fact, it is often not satisfied), the resolution cannot reach the given value. Imaging, and the comparison of images formed by using a true color confocal microscope, in which the red circles are doped with different compounds, you can clearly see the image formed by a monochromatic laser confocal microscope, grayscale alone cannot accurately reflect Among them, the difference between the compound doping, but the true color confocal can clearly distinguish their difference.
