Why electron microscopes can't replace light microscopes
The electron microscope uses the principle of electron optics, replacing the light beam and optical lens with an electron beam and electron lens, so that the fine structure of the material is imaged under very high magnification. It has been far better than the optical microscope, but the electron microscope needs to work in vacuum conditions, so it is difficult to observe living organisms, and the irradiation of the electron beam will also make the biological samples irradiation damage, so it can not completely replace the optical microscope, and their cost is not the same, suitable for the work of the scope of the work is also not the same. I hope my answer can help you.
Electron microscope can not completely replace the optical microscope for the following reasons:
1. electronic microscope belongs to the optical microscope based on the addition of CCD, display or computer accessories, which can only be said to be a video microscope, the entire imaging process, CCD instead of the human eye. Because the video imaging, electronic magnification is false amplification, and on the pixel, light-sensitive effect and other factors, with the human eye is too big a difference, so with the visual microscope effect is too big a difference;
2. There is a most important reason, CCD belongs to the plane imaging, and the human eye, especially binocular observation, will produce a strong sense of three-dimensional, which is the two contrast depth of field effect is too large;
3. Electron microscope is mostly expressed as an electron scanning microscope, this microscope effect will be much better than the general effect of optical microscopy, but because of the high price, so few applications and industry.
