Why Electron Microscopes Cannot Replace Light Microscopes
Electron microscopes use the principle of electron optics, replacing light beams and optical lenses with electron beams and electron lenses, so that the fine structures of substances can be imaged at very high magnifications. Although its resolving power is far better than that of optical microscopes, electron microscopes are difficult to observe living organisms because they need to work under vacuum conditions, and the irradiation of electron beams will also damage biological samples, so they cannot completely replace optical microscopes. Microscopes, and their cost is not the same, the scope of work is not the same. Hope my answer can help you.
Electron microscopes cannot completely replace optical microscopes for the following reasons:
1. Electron microscopes are optical microscopes with CCDs, display screens or computer accessories added to them. This can only be called a video microscope. During the entire imaging process, CCDs replace the human eye. Because in video imaging, electronic magnification is a virtual magnification, and in terms of pixels, photosensitive effects and other factors, it is too different from the human eye, so the effect is too different from that of a visual microscope;
2. There is another most important reason, CCD belongs to planar imaging, and human eyes, especially in the case of binocular observation, will produce a strong three-dimensional effect, which is the reason why the contrast effect of the two is too large;
3. Electron microscopes are mostly expressed as scanning electron microscopes. The effect of this kind of microscope is much better than that of ordinary optical microscopes, but because of its high price, it is rarely used in industry.
