Why is the imaging quality of laser confocal microscope better?
The principle of laser confocal microscope is that the light beam emitted by LED light source passes through a porous disk and an objective lens and then focuses on the surface of the sample. After that, the light beam is reflected back to the measurement system by the sample surface. When passing through the pinhole on MPD again, the reflected light will only keep the focused spot. Finally, the beam is reflected by the beam splitter and imaged on the camera.
1. Laser scanning technology is used in laser confocal microscope. Compared with the broad-spectrum light source of traditional microscope, laser scanning technology can accurately locate and focus on the specific area of the sample, thus improving the resolution and accuracy of imaging. At the same time, laser scanning technology can eliminate the scattering and background signal in the sample, thus improving the contrast of imaging. At the same time, the monochromaticity of laser makes the imaging clearer.
2. The laser confocal microscope has a large optical aperture (the ability of the microscope to receive the light emitted by the sample) and a high numerical aperture objective (the magnification of the lens), which makes the imaging clearer and more detailed.
3. Laser confocal microscope uses a photomultiplier tube with high sensitivity, which can show high sensitivity to weak fluorescence signals, and can also eliminate background noise by narrowing the excitation range and using optical slices. * * Equipped with high-sensitivity photodiode detector, the laser confocal microscope can quickly and accurately detect optical signals and convert them into electrical signals.
Different from traditional optical observation, photodiode detector can detect a single photon, making imaging more sensitive and accurate. This high-sensitivity detector can also obtain clear images under low light intensity conditions.
