9 Important Factors Affecting Microscope Imaging
1. When using a microscope, it is important to make the object under inspection more standard. Such as: whether the slice thickness is too thick, whether the cover glass meets the national standard, etc.
2. The microscope objective lens can be divided into about 6-8 levels according to the grade, and the most commonly used one is the plan achromatic objective lens. If the lens grade is too low, the image quality will decrease. Therefore, it is recommended to choose an objective lens with a grade above the flat field.
3. The aperture diaphragm of the condenser should match the numerical aperture of the objective lens as much as possible. In order to get the best image resolution.
4. The focusing system is an important part of the mechanical part, and the focusing accuracy is also very important. For beginners in biology or operators who are new to the microscope, skilled use of the focusing system can quickly find and focus the image and improve work efficiency.
5. The stage often moves in X and Y when observing slices. For example, the double-layer mechanical mobile platform has good precision and stability, and can provide good imaging quality when observing microscopic images.
6. The aspherical light collecting lens can provide very uniform illumination light, and the contrast of the observed microscopic image can be improved.
7. The field diaphragm should be opened to a suitable position according to the observed objects and the magnification of the objective lens. If it is opened too large or too small, the brightness of the field of view of the microscopic image will be affected.
8. If the lighting system adopts a lighting device with low temperature and high color temperature, what is observed is a microscopic image with extremely high resolution and sharpness.
9. Using Kolar lighting system, it can adjust the optical center image. All scientific research microscopes use this system, which provides micrographs for microscopic examination and professional digital microscopic imaging system.
In order to ensure that the microscope has a good imaging effect, the above nine aspects should be paid special attention to when using the microscope in the laboratory.
