9 Important Factors Affecting Microscopy Imaging
The use of microscopes in the laboratory should pay attention to many aspects. The imaging of microscopes has always been the focus of researchers. The factors that affect the imaging of laboratory microscopes are mainly in the following 9 aspects.
1. When using a microscope, it is important to make the object under inspection more standard. Such as: whether the thickness of the slice is too thick, whether the cover glass meets the national standard, etc.
2. The microscope objective lens can be divided into about 6-8 grades according to the grade, the most commonly used is the plan achromatic objective lens. If the lens grade is too low, the image quality will be degraded. Therefore, it is recommended to choose an objective lens with a flat field or higher.
3. The condenser aperture diaphragm should match the numerical aperture of the objective lens as much as possible. to get the best image resolution.
4. The focusing system is an important part of the mechanical part, and the focusing accuracy is also very important. For beginners in biology or operators who are new to microscopes, skilled use of the focusing system can quickly find images and focus, improving work efficiency.
5. The stage often moves X and Y when observing slices. For example, the double-layer mechanical moving platform has good precision and stability, and can provide good imaging quality when observing microscopic images.
6. The aspherical concentrator can provide a very uniform illumination light, and the observed microscopic image contrast.
7. The field diaphragm should be opened to an appropriate position according to the different objects under inspection and the magnification of the objective lens. If it is opened too large or too small, the field of view brightness of the microscopic image will be affected.
8. If the illumination system adopts the illumination device with low temperature and high color temperature, the microscopic image with extremely high resolution and sharpness is observed.
9. Using the Kola lighting system, the optical center image can be adjusted. Scientific research-level microscopes use this system to provide micrographs for microscopy and professional digital microscopic imaging systems.
In order to ensure that the microscope has a good imaging effect, the above 9 aspects should be paid special attention to when using the microscope in the laboratory.
