Advantages of laser confocal microscopy over optical microscopy
(1) Laser confocal microscope The image is recorded in the form of electrical signals, so a variety of analogue and digital electronic techniques can be used for image processing.
(2) The laser confocal microscope uses a confocal system that effectively eliminates the interference of light signals outside the focal point, improves the resolution, significantly improves the breadth and depth of the field of view, and makes it possible to make damage-free optical sections and achieve three-dimensional spatial positioning.
(3) As the laser confocal microscope can collect and record the detection signals at any time, it opens up a new way for life sciences to observe the structure of living cells as well as the biological changes of specific molecules and ions.
(4) In addition to the imaging function, laser confocal microscope also has image processing function and cell biology function. The image processing function includes optical sectioning, three-dimensional image reconstruction, cell physical and biological determination, fluorescence quantification, localisation analysis and real-time quantitative determination of ions. Cell biology functions include sorting of adherent cells, laser cell fibre surgery and light trap technology, and fluorescence bleaching recovery technology.
Convex lens imaging laws in metallurgical microscopy:
1. in metallurgical microscope, when the object is located outside the lens object two times the focal length, then in the elephant side two times the focal length, outside the focus of the formation of reduced inverted image;
2. when the object is located in the lens object two times the focal length, then in the image side of the two times the focal length of the formation of the same size of the inverted image; this imaging is very important to the optical path of the metallurgical microscope.
3. when the object is located in the lens object two times the focal length, outside the focus, then in the elephant side two times the focal length outside the formation of magnified inverted image. 4;
4. when the object is located in the focal point of the lens object, the elephant side can not become an image; this is also an important factor affecting the metallurgical microscope image.
5. when the object is located within the focal point of the lens object, the elephant side of the formation of no image, but in the same side of the lens object than the object far away from the position of the formation of magnified upright virtual image.
