Applications in Biology - Laser Scanning Confocal Microscope
1. Suitable for almost all fields related to cell research, including cell biology, cell physiology, neurobiology, and neurophysiology.
2. Conduct non-destructive real-time observation and analysis of live cells, and conduct research that combines morphology and function. Non damaging, reliable, and excellent repeatability in cell detection; Data images can be output in a timely manner or stored for a long time.
3. Continuous cross-sectional scanning of live cells and tissues or cell tissue sections can obtain detailed two-dimensional and three-dimensional structures of individual cells, groups of cells, or observed local tissues (including cell specific structures such as cytoskeleton, chromosomes, organelles, and cell membrane systems, as well as deep structures of samples) and complete three-dimensional images (such as analyzing changes over time, i.e. four-dimensional images, or images that vary with fluorescence wavelength to achieve more dimensional images). Locate the spatial position of organizational cells and other structures that need to be observed, and perform real-time dynamic observation, analysis, and recording; Qualitative, quantitative, timed, and positional distribution of analysis.
4. Observation of cellular biomass, membrane labeling, cell tracing, substances, reactions, receptors or ligands, nucleic acids, etc. in live cells or sliced specimens labeled with fluorescent probes; Multiple substance labeling can be performed simultaneously on the same sample for simultaneous observation.
5. Intracellular ion fluorescence labeling, single or multiple labeling, to detect the ratio and dynamic changes of intracellular concentrations such as pH and sodium, potassium, calcium, and magnesium ions;
6. Measurement of cell membrane potential, detection of free radicals, etc;
7. Conduct targeted fluorescence bleaching recovery experiments, combined with fluorescence loss experiments in fluorescence bleaching, to study intercellular communication and the movement of other intracellular substances (molecules, etc.); In time scanning experiments and photo bleaching (photo quenching) experiments, data and images from each channel can be simultaneously output and converted. Conduct fluorescence resonance energy transfer experiments to study the movement and interactions of molecules and ions within cells through changes in fluorescence wavelength.
8. It has very safe (spatial positioning, quantification, wavelength, time), sensitive, fast, and can complete the separation, observation, and analysis of various wavelength images of cell tissue multi fluorescence labeling (even if the emission wavelengths are very close, such as multi fluorescence with a difference of only a few nm) at the same time, as well as online measurement and analysis functions such as co localization of multi fluorescence labeling
