Basic operating steps for microscopes and digital cameras
1. Warm up the fluorescent light source. (For fluorescence microscopy)
2. Without placing glass slides, adjust the shooting lever to the digital camera for white balance.
3. Adjust the shooting lever to the eyepiece observation position.
4. Adjust the distance between the pupils
5. Adjust parallax
6. Search for targets in a bright field of view and low magnification objective, focusing
7. Change to a suitable objective lens and focus.
8. Adjust the brightness of the light source
9. Adjusting the spotlight
10. Adjust the filter or aperture as needed
When a clear image is visible in the eyepiece, adjust the microscope to a digital camera as follows:
11. Adjust the shooting lever to transfer the image to the digital camera.
12. Check for focus under a bright field of view. If it is not in focus, turn the fine focus knob to focus. At this point, it should be a bright field of view, keeping the camera in a high frame rate state.
13. Adjust the color of the camera, and you can manually balance the white balance as needed, or manually adjust the gain values of red, green, and blue.
14. Take photos,
When shooting, with a bright field of view, the CMOS camera should lower the gain value and adjust the saturation as needed.
When taking fluorescent photos, appropriate gain values should be selected according to recommendations, and the camera frame rate should be lowered to ensure low noise in the captured photos.
If better fluorescent images need to be taken, the camera should be optimized for microscopic photography, capable of cooling and reducing noise. In addition, the frame rate of the camera can be adjusted to reduce noise.
