Microscope debugging and usage methods
After understanding the names, structures, and functions of the main components of the microscope, in order to better utilize the various functions of the microscope, improve work efficiency, and ensure the best results during microscopic observation and imaging, users must understand and master the correct debugging and usage methods of the microscope. Especially in the new generation of microscopes, they have multiple functions and can be observed using various microscope inspection methods. Therefore, the correct debugging and usage methods are particularly important. Taking the universal research microscope as an example, briefly describe the debugging and usage methods.
1. Adjustment of the microscope lighting system
In order to provide a uniform and sufficient illumination of the microscope's field of view, it is necessary to adjust the lighting system during the initial installation and debugging of the microscope. This is an important means and basic requirement for correctly using the microscope and obtaining correct and achievable results. In addition, correctly mastering the adjustment of the lighting system is a necessary step after replacing the light source bulb during the use of the microscope, and it is also a necessary means to regularly check the performance of the microscope during daily use. The adjustment of the microscope lighting system mainly includes the following four aspects:
(1) Preliminary adjustment of the lighting source chamber outside the microscope
① Firstly, open the outer shell of the lamp chamber and press the spring clamp to install the halogen bulb into the socket. During installation, avoid direct contact with the bulb with your fingers (using a soft cloth or paper to isolate it), to avoid leaving fingerprints and other dirt on the bulb, which may affect its service life.
② Place the lamp room on the desktop, turn on the power, and use a dedicated screwdriver to adjust the focusing knob hole of the lamp (marked with "←→") to project the filament onto a wall 1-2m away, and adjust the image of the filament to be clear; Then adjust the high and low position of the lamp to adjust the filament hole (marked with "-") so that the filament position is appropriate; Adjust the left and right positions of the lamp to adjust the screw holes (marked with "-"), so that the left and right positions of the filament are suitable.
(2) The purpose of checking and correcting the position of the light emitting body (filament) in the microscope is to align the image end of the light emitting body into the field of view of the objective lens, ensuring that the field of view of the microscope is fully and uniformly illuminated from the perspective of the light source. This is a prerequisite for adjusting the Kuhler lighting system. Basic tools required: The alignment telescope was already equipped when purchasing a microscope.
① Unplug the ground glass sleeve from the lamp compartment and install the lamp compartment back onto the microscope;
② Select 10 × Objective lens, turn on the light source program to find the sample and focus clearly, then switch to 40 × The objective lens focuses the sample clearly (40 × The objective lens can clearly see the full view of the filament;
③ Open the aperture aperture aperture and field of view aperture of the condenser to *;
④ Remove one of the eyepieces, replace it with a centering telescope, grab the white part, and with the other hand, retract the black eyepiece to see the filament image in the field of view;
⑤ If the position of the filament is not suitable, adjust the "-" hole to adjust the filament image horizontally, adjust the "-" hole to adjust the filament image vertically, until the filament image is adjusted to a circular image that exactly fills the aperture of the objective lens;
⑥ After the adjustment is completed, insert the ground glass sleeve back into its original position, remove the centering telescope, and replace it with the eyepiece for the next adjustment. The adjustment of the lighting source lamp chamber outside the microscope and the calibration of the position of the light emitting body inside the microscope mentioned above only need to be carried out during the initial installation, debugging, and replacement of the light bulb of the microscope. It is not allowed to adjust and move the microscope randomly during normal use. In case of confusion, you can follow the above steps to restore it to its original state.
