Biological microscopy performed on the volume of the tissue blocks
Biological microscopes Microscopes with a condenser can move the condenser up and down to make its brightness moderate, and also can change the aperture of the variable light elaboration in order to achieve a moderate 9b brightness. If the light is sunny, the condenser can be moved upward and the aperture of the variable light can be enlarged. If the light is too strong, then the spotlight mirror can be appropriate down, the aperture of the variable light leap for appropriate reduction. If in this case still feel blinding, then you can choose the appropriate filter placed in the bracket under the spotlight. This can be obtained to make you satisfied with the brightness. Of course, in the adjustment of the upper and lower positions of the condenser lens can vary the size of the aperture of the light reading and the selection of suitable filters, that is after a certain period of practice and experience.
Biological microscope A very important issue is in the process of sampling and separation of cells, freeze-drying and resin embedding (FD) after freezing ultra-booklets, freeze-drying after the fine run of each part of the 65 elemental composition content must be handled very carefully, and must not damage the cells to be observed and analysed. Because the x-ray micro-area analysis is not only a lot of steps, and the cost is very high, if after a long time and multiple steps of processing, the analysed cells are damaged cells or dead cells, to draw the wrong conclusions is very regrettable. For example, the cardiomyocytes isolated by the collagenase treatment have two morphologies, one is long rod-shaped and the other is round. The latter are dying cells that were damaged during the cell isolation process.
Biological microscope The myofibre can be placed on a special rack, so that the contraction of this myofibre reaches a certain time phase when it needs to be fixed, and then immediately activate the nozzle, so that liquid propane is sprayed to the myofibre, so that it is quenched and cold fixed. Then the muscle fibre is taken out together with the rack and put into liquid nitrogen. If fixing blood cells or isolated cells, first low-speed centrifugation to make the cells concentrated, the cells will be transferred to a good thermal conductivity of the silver tube, the tube into the liquid propane carcass cold fixed. Hall laboratory fixed rat pancreas, the first two pieces of steel with liquid helium (or liquid nitrogen) pre-cooling, with a clamp clamped the two pieces of copper will be placed in front of and behind the pancreas, so that the aunt of the gland quenching cold fixation. Tissues or cells can be stored for a long time by transferring them to liquid nitrogen after quenching and fixing.
Biomicroscopy In addition to the currently *promoted* method of frozen ultrathin sections, a further deposition technique used by scientists is described here. A substance is added prior to analysis so that it forms a deposit with the component to be analysed in order to immobilise it, and the deposit is then analysed. For example, oxalate and pyroantimonate are commonly used to deposit ca in myocytes; the former is not sensitive enough for low concentrations of ca in the cytoplasm; pyroantimonate has a higher sensitivity and forms electronically dense deposits with free ca in the plasma, but pyroantimonate also forms deposits with sodium, manganese, barium, and iron, which have a poorer specificity. The process of specimen preparation was similar to that of ordinary transmission electron microscopy ultrathin section specimens, with the difference that 3% potassium pyroantimonate (phosphate buffer, pH 7.6) was fixed for 6 hours before fixation, and on stained ultrathin sections of the muscle, a black precipitate was seen in the midline of the A and 2 bands of each myonectomy section, and then the unstained sections were used for X-ray micro-area analysis. Diaminobenzidine tetrahydrochloride (DAB) has been used to precipitate haemoglobin-containing material, among others. This kind of precipitation reaction of histochemistry and x-ray micro-area bridge can be considered to be used in laboratories that do not have the condition of frozen ultra-thin sections. The peak heights of the elements to be analysed can be used for semi-quantitative analysis.
