Comparison between Stereo Microscope and General Biological Microscope
Stereo microscope is a kind of optical microscope with visible light as light source, and it is a compound microscope that can observe objects with both eyes and thus have stereoscopic vision. The basic structure is an optical system to ensure imaging and a mechanical part to install the optical system.
Comparison between Stereo Microscope and General Biological Microscope
The characteristics and usage of the stereo microscope are not inverted. Usually, the light shines on the specimen from the oblique top of the object, so the surface of the specimen is observed. Compared with the general biological microscope, the stereoscopic microscope has a larger depth of focus, and can place larger samples, such as stems, leaves, flowers and other plant organs, and observers can also perform anatomical operations under the stereoscopic microscope. Its magnification is the product of objective lens, zoom objective lens and eyepiece. However, the objective lens is generally not used. Once the objective lens is used, the observation field of view is smaller, the focal depth is smaller, and the light in the field of view is dimmed.
Characteristics of stereomicroscope
1. The left and right beams in the binocular lens barrel are not parallel, but have a certain included angle-volume angle (generally 12-15 degrees), so the imaging has a three-dimensional sense;
2. The image is upright and easy to operate and dissect, because the prism under the eyepiece turns the image upside down;
3. The working distance is very long and the field of view is large.
4. The depth of focus is large, which is helpful to observe the whole layer of the inspected object.
How to use stereomicroscope
1. According to the color of the object, select one side of the workbench in black and white, and put the object to be observed in a glass slide or a Petri dish and then put it on the workbench.
2, choose the appropriate magnification, put on the required eyepiece (10 x or 20 x). If it is observed below 80×, the 2× large objective lens can be removed, and its effective working distance is 87 mm. If it is added with the 2× large objective lens, the magnification can reach 160×, and the effective working distance is 26 mm. By adjusting the working distance, the handwheel can be loosened, and it can be achieved by pulling out or pressing in the movable pillar.
3. During operation, move the object to the center of the workbench, rotate the lifting handwheel, so that the left eyepiece can see a clear object image. If the image of the right eyepiece is not clear, you can rotate the eyepiece focusing ring to get the same clear object image as the left eyepiece, so that you can see a clear object image with stereoscopic effect, and the focusing work is basically completed. In order to get the appropriate magnification, we can change the magnification of the zoom objective by rotating the magnification adjusting ring. The magnification of the zoom objective can be read on the reading ring. When necessary, the right-angle prism group can be adjusted to change the distance between eyepieces to suit the pupil distance above the observer's eyes. Loosen the fastening screw to make the microscope rotate at any position around the axis.
