Correct use and maintenance methods of microscopes
1. Placement
Hold the mirror arm with your right hand and the mirror base with your left hand, keeping the mirror body upright. The desktop should be clean and stable, and a window facing or well lit area should be chosen. The single tube is usually placed on the left side, 3-4 centimeters away from the table edge.
2. Cleaning
Check if the microscope is faulty and clean. The mechanical part of the mirror body can be wiped with a clean soft cloth. The lens should be wiped with lens wiping paper. If there is glue or dirt, a small amount of xylene can be used to clean it.
3. Aiming light
Raise the lens tube to a distance of 1-2 centimeters from the stage, and align the low-power lens with the light hole. Adjust the aperture and reflector. When the light is strong, use a flat mirror. When the light is weak, use a concave mirror. The reflector should be rotated with both hands.
If a microscope with a light source is used, the secondary step can be omitted, but a knob for adjusting the brightness is required.
4. Install specimen
Place the glass slide on the stage, ensuring that the side with the cover glass is facing upwards. Fix the glass slide with a spring clip and rotate the knob of the platform shifter to align the material to be observed with the center of the light hole.
5. Focusing
When focusing, first rotate the coarse focusing knob to slowly lower the lens barrel, and carefully observe from the side until the objective lens is close to the glass specimen. Then, observe with the left eye from the eyepiece, and rotate the coarse focusing knob with the left hand to lift the lens barrel until the specimen image is clearly visible. Then, use the fine focusing knob to recall clarity.
Operation note: Do not focus directly under a high-power lens; When the lens tube descends, the distance between the lens tube and the specimen should be observed from the side; To understand the critical value of object distance.
If a binocular microscope is used, if there is a difference in the binocular vision of the observer, the reliable vision adjustment circle should be used for adjustment. In addition, the two cylinders can be relatively translational to adapt to the distance between the operator's eyes.
6. Observation
If using a single tube microscope, open both eyes naturally, observe the specimen with the left eye, record and plot with the right eye, and adjust the focal length with the left hand to make the object clear and move the specimen's field of view. Record and draw with the right hand.
During microscopic examination, the specimen should be moved in a certain direction until the entire specimen is observed, so as not to miss or repeat the examination.
