Difference between two-photon microscope and laser confocal microscope
Difference between fluorescence microscope and laser confocal microscope
Laser confocal microscope is a set of observation, analysis and output system which uses laser as light source, adopts conjugate focusing principle and device on the basis of traditional optical microscope, and uses computer to process the digital image of the observed object. The main system includes laser light source, automatic microscope, scanning module (including confocal optical path and pinhole, scanning mirror and detector), digital signal processor, computer and image output equipment (display, color printer), etc. Through the laser scanning confocal microscope, the observed samples can be scanned and imaged. Therefore, the three-dimensional spatial structure of cells can be observed and analyzed without damage.
At the same time, laser scanning confocal microscope is also a powerful tool for dynamic observation of living cells, multiple immunofluorescence technique and ion fluorescence labeling observation, which accurately analyzes the essence of the spectrum and distinguishes the signals of different labels with highly overlapping emission spectra.
Most importantly, for multicolor fluorescent dyeing, it can completely eliminate the influence of fluorescent cross-color, and at the same time minimize the loss of fluorescent signal of samples. These are all beyond the reach of ordinary light mirrors.
Uses of various optical microscopes
The fluorescence microscope uses the fluorescence emitted by the specimen to observe the object;
Stereo microscope can be used to observe the stereoscopic image of objects, etc.
The projection microscope can project the object image on the projection screen for several people to observe at the same time;
Inverted microscope is used for cell culture, tissue culture and microbial research;
The phase contrast microscope is used to observe colorless and transparent specimens;
For example, a dark field microscope is used to observe the movement of bacteria and spirochetes.
