Differences and similarities between fluorescence microscopy and optical microscopy
Fluorescence microscope is a type of optical microscope
Fluorescence microscope is a device that uses ultraviolet light as a light source to illuminate the object being tested, causing it to emit fluorescence, and then observe the shape and position of the object under the microscope. Fluorescence microscopy is used to study the absorption, transportation, distribution, and localization of substances within cells.
Some substances in cells, such as chlorophyll, can emit fluorescence after being exposed to ultraviolet radiation; Some substances themselves may not emit fluorescence, but if stained with fluorescent dyes or fluorescent antibodies, they can also emit fluorescence under ultraviolet radiation. Fluorescence microscopy is one of the tools for qualitative and quantitative research on these substances.
There are the following differences between fluorescence microscopy and ordinary microscopy:
1. The lighting method is usually a drop type, where the light source is projected onto the sample through the objective lens;
2. The light source is ultraviolet light, with a shorter wavelength and higher resolution than ordinary microscopes;
3. There are two special filters, the one in front of the light source is used to filter out visible light, and the one between the eyepiece and objective is used to filter out ultraviolet rays to protect the human eye.
Fluorescence microscopy is also a type of optical microscope, with the main difference being the different excitation wavelengths between the two. This determines the differences in structure and usage between fluorescence microscopy and ordinary optical microscopy.
Fluorescence microscopy is a fundamental tool for immunofluorescence cytochemistry. It is composed of main components such as light source, filter plate system, and optical system. It is to use a certain wavelength of light to excite the specimen and emit fluorescence, and to observe the fluorescence image of the specimen through an objective and eyepiece system amplification.
