Differences Between Fluorescence Microscopes and Ordinary Microscopes
Fluorescence microscope uses ultraviolet light as a light source to illuminate the object being inspected, causing the object to emit a light source, and then observe the object under the microscope. Mainly used for immunofluorescence cells, it is composed of a light source, filter plate system, and optical system to observe the fluorescence image of the sample through magnification of the eyepiece and objective lens. Let's take a look at the differences between this fluorescence microscope and a regular optical microscope.
1. Looking at the lighting method
The illumination method of a fluorescence microscope is generally a falling beam type, which means that the light source is projected onto the test sample through the objective lens.
2. Looking at the resolution
Fluorescence microscopes use ultraviolet light as a light source, with a shorter wavelength but higher resolution than ordinary optical microscopes.
3. Differences on the filter
Fluorescence microscope uses two special filters, one in front of the light source to filter out visible light and the other between the objective lens and eyepiece to filter out ultraviolet light, which can protect human eyes.
Fluorescence microscope is also a type of optical microscope, mainly due to the short wavelength excited by fluorescence microscope, which leads to differences in the structure and use of fluorescence microscope and ordinary microscope. Most fluorescence microscopes have good ability to capture weak light, so their imaging ability is also good under extremely weak fluorescence. In addition, with the continuous improvement of fluorescence microscopes in recent years, the noise has also been significantly reduced. Therefore, more and more fluorescence microscopes are being applied.
