Effects of Active Substances on Calcium in Hilar Cells Observed by Laser Scanning Confocal Microscopy
The change of ionized calcium concentration in cells is the material basis of calcium signal as the second messenger of cell communication, so it is very important to measure the concentration of ionized calcium in cells in resting state and activated state. In the past, people can only use microinjection method to observe ionized calcium in a few large cells by using bioluminescent protein, metal filling indicator, ca' selective microelectrode and other methods. Since the 1980s, with the combined application of ca2'-specific fluorescent indicators and microfluorescence photometers, people began to study ca" signals through cell-free sodium (: az' determination methods. Until recently, the application of laser scanning confocal microscopes has greatly accelerated people's research on ionized calcium in single cells and its subcellular distribution. Using Leica laser scanning confocal microscopes and ca"-specific fluorescent indicators f1Mo-3, it is possible to observe the effects of various biologically active substances on ionized calcium in 1.I-PN cells.
The c-PK1 cell on I is a cell line derived from pig kidney tubule 1: skin, which has been recognized by the international academic community. It shares some of the transport functions with the proximal tubule epithelium, yet its response to stimuli is similar to that of the thick ascending limb of Heinz mix. Therefore, PR1 cells on N are often used instead of proximal tubule epithelial cells to study the transport of substances, and instead of Heinz mixed with cultural section epithelial cells to study its response to hormones. There are also people in the world who use I'I'c-PK1 cells to study intracellular calcium messengers in renal tubular epithelial cells. u, c—PK1 cells exchange 50% of the total cellular calcium with the outside world within 20 mm, and the remaining cellular calcium exchanges with the outside world more aggressively, which is called the invaded exchange calcium pool. In the nucleus, the Golgi apparatus, and other regions of the cytoplasm, there are two parts, the fast-exchange calcium pool and the slow-exchange calcium pool.
