Q: Why do I have to use immersion oil for the 100x objective?
A: In order to get strong magnification, the lens must be cut very small. Therefore, it is important to let as much light as possible pass through the lens so that the observer can see the object in question. The oil changes the refraction of the light, so the light passes through the lens instead of being bent and scattered in all directions.
Q: Which is better: a planar achromatic microscope or an achromatic microscope?
A: Both microscopes are ideal for viewing specimens in the center of the viewing area. However, if bacteria must be counted or the shape of bacteria must be distinguished anywhere in the field of view, a plan achromatic microscope is more suitable. These microscopes have a lens system that compensates for distortions at the edges of the viewing area so that the specimen appears extremely sharp.
Q: How do I know the total magnification of the content being viewed?
A: The first thing to determine is the magnification of the IOL; that is the lens closest to the viewer. The magnification of this lens will be etched into the metal. The standard magnification of the eyepiece is 10x. Then you have to find the magnification of the objective. That is the lens closest to the sample. This number will also be engraved into the target. These two magnifications are then multiplied together to obtain the total magnification. For example, if you have a standard 10x eyepiece and use a 40x objective, the total magnification will be 400x.
