Guidelines for using an optical microscope
(1) The mirror seat should be about 6 to 7 cm away from the table edge throughout the experiment, and the microscope should be set up on the table in front of the seat with a small leftward tilt.
(2) Switch on the light source, then set the light's intensity to the proper level.
(3) Turn the rotating objective lens so that the low power lens is directed at the stage's light hole. Adjust the lens to be between one and two centimeters away from the stage, then gaze through the eyepiece with your left eye. Finally, adjust the height of the condenser and the aperture diaphragm to the widest setting so that the light entering the condenser enters the lens barrel and illuminates the field of view.
(4) Center the observed portion of the slide on the stage so that the light hole is in the center of the slide. Clamp the slide with the specimen holder.
(5) Start by using a low power lens (10X objective, 10X eyepiece). To begin observation, raise the stage by turning the coarse focusing handwheel. The objective lens will then progressively approach the glass slide.After that, turn the coarse focus adjustment knob so that the stage is gradually lowered, and after a while, a magnified image of the material on the slide can be seen. It is important to develop the habit of observing with both eyes open while using a microscope so that you can look at the drawing with the right eye while observing.
(6) Slowly adjust the stage's moving handle if the item picture visible in the field of view does not adhere to the experimental conditions (it deviates from the field of view). When adjusting, keep in mind that the glass slide moves in the exact opposite direction to the object picture visible in the field of view. You can adjust the micro focus handwheel till the object image is clear if the picture of the item is not very clear.
(7) Before switching to a high-power objective lens for observation, you should move the area of the object image that needs to be enlarged for observation to the center of the field of view. This is because when you switch from a low-power to a high-power objective lens for observation, the object image's field of view is reduced. plenty of).The low magnification objective lens and the high magnification objective lens are often essentially in focus for microscopes with regular functions. You should be able to see the object image with the high magnification objective lens when using the low magnification objective lens to observe well, although the object image isn't always extremely clear. To change the focus, turn the micro focus. To adjust, turn the handwheel.
(8) Once you've switched to a high-magnification objective lens and can clearly see the image of the object, you can adjust the condenser's height or size to ensure that the light is sufficient. Generally speaking, the field of view should be a little bit smaller when a low-magnification objective lens is replaced with a high-magnification objective lens for observation. You must adjust the light intensity as it grows darker.
(9) Following the observation, the objective lens should be removed from the light hole, the aperture diaphragm should be set to the maximum setting, the stage should be slowly lowered, and the parts should be examined for damage (in particular, make sure the objective lens is in good condition). After examination and treatment, it can be packed (if it has water or oil stains, wipe them off with lens paper).
