How does a microscope's magnification factor work?

Jun 08, 2024

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How does a microscope's magnification factor work?

 

Many laboratories are using microscopes, but they are not familiar with the relevant professional knowledge of microscopes. They only know how to operate them, but may not be very clear about some basic knowledge. So today, we will talk about how to calculate the magnification of microscopes?


Perhaps some people may say that this is not a very simple problem, but in reality, it is still a bit complicated.
Firstly, let's give an example: when the magnification of the stereo microscope eyepiece is 10 times, the magnification range of the variable magnification is 0.7X-4.5X, and the additional objective is 2X. The optical magnification of this microscope is 10 times 0.7 times 2, resulting in a low magnification of 14 times and a high magnification of 10 times 4.5 times 2, which is 90 times. Therefore, the total optical magnification of this stereo microscope is 14 to 90 times. Of course, this is only the actual magnification of the microscope host. Next is the digital magnification of the microscope.


For example, if the size of the monitor is 17 inches and a 1/3 microscope camera is used, the digital magnification of the microscope camera is 72 times compared to the table below. The calculation formula for the digital magnification of the microscope is as follows: based on the configuration of the stereo microscope, the variable magnification is 0.7X-4.5X, and the additional objective is 2X. The camera eyepiece is 1 (if the camera eyepiece has no multiple, it does not need to be included in the calculation). According to the formula: objective X camera eyepiece magnification X digital magnification, the digital magnification is 0.7 times 2 times 1 times 72, which is 100.8 times, and the digital magnification is 4.5 times 2 times 1 times 72, which is 648 times. The range of digital magnification is 100.8 times to 648 times.


In this way, two formulas will appear:
1. Total optical magnification=magnification of the eyepiece X magnification of the objective lens


2. Digital total magnification=objective lens X camera eyepiece magnification X digital magnification
This formula is suitable for any microscope, whether it is a metallographic microscope, a biological microscope, etc

 

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