How to Adjust Olympus Microscope Illumination Optical System
In order to ensure that the field of view of the Olympus microscope can be evenly and fully illuminated, the illumination optical system must be adjusted when the microscope is first installed and debugged. This is an important means and guarantee for correct use of the microscope and correct results. Basic requirements. In addition, correctly mastering the adjustment of the illumination optical path system is a necessary step after replacing the light source bulb during the use of the microscope, and it is also a necessary means to check the performance of the microscope from time to time during daily use. The adjustment of the microscope illumination optical path system mainly includes the following four items:
1. Preliminary adjustment of Olympus microscope illumination light source lamp chamber outside the microscope
2. First open the shell of the lamp chamber, press the spring clip to put the halogen bulb into the socket, and avoid direct contact with the bulb with your fingers during installation (you can use soft cloth or paper to separate it), so as not to leave fingerprints and other dirt on the bulb, which will affect the light bulb. The lifetime of the light bulb.
3. Put the lamp house on the table, after turning on the power, use a special screwdriver to adjust the focus knob hole of the lamp (marked with "←→"), so that the filament is projected on the wall 1-2m away, and the filament is imaged Adjust it to clear; then adjust the height of the lamp and adjust the thread hole (marked with "──") to make the filament position appropriate; then adjust the left and right position of the lamp to adjust the screw hole (marked with "──") to make the left and right position of the filament suitable.
4. The purpose of checking and correcting the position of the light source illuminant (filament) in the microscope is to adjust the image end of the illuminant correctly into the field of view of the objective lens, and to ensure that the field of view of the microscope is fully and fully controlled from the perspective of the light source. Uniform lighting, which is a prerequisite for adjusting the Kuhler lighting system. Basic tools needed: The centering telescope is equipped when the microscope is purchased.
5. Unplug the frosted glass sleeve in the lamp house, and put the lamp house back on the microscope;
6. Select the 10× objective lens, turn on the light source program to find the sample and focus clearly, and then use the 40× objective lens to focus the sample clearly (40× objective lens can see the whole picture of the filament);
7. Open the aperture diaphragm and field diaphragm of the condenser to the maximum;
8. Unplug one of the eyepieces, replace the centering telescope, grasp the white part, and stretch the black eyepiece with the other hand, and you can see the filament image in the field of vision;
9. If the position of the filament is not suitable, adjust the "──" hole to adjust the filament image in the horizontal direction, and adjust the "──" hole to adjust the filament image in the vertical direction until the filament image is adjusted to just fill the aperture of the objective lens the round image of light;
10. After the adjustment is completed, insert the frosted glass sleeve back to its original position, unplug the centering telescope, and replace the eyepiece for the next adjustment. The above-mentioned adjustment of the illumination light source lamp chamber outside the microscope and the verification of the position of the light source luminous body inside the microscope only need to be carried out when the microscope is first installed and debugged and when the bulb is replaced, and the microscope cannot be adjusted randomly when using the microscope. In case of confusion, it can be adjusted back to the original state according to the above steps.
