Small details and precautions in microscope operation

Jul 11, 2023

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Small details and precautions in microscope operation

 

Stereo microscopes are widely used in various sectors of industry, agriculture and scientific research because of their many advantages. If there are some problems during use, you can solve them yourself according to the actual situation. According to the actual usage, the common faults are: the field of view is blurred or there is dirt. The possible reasons are dirt on the specimen, dirt on the surface of the eyepiece, dirt on the surface of the objective lens, and dirt on the surface of the working plate.


According to the actual situation, it can be solved by cleaning the dirt on the surface of the specimen, eyepiece, objective lens and working plate. The possible reason for the misalignment of the two images is that the interpupillary distance adjustment is incorrect, and measures to correct the interpupillary distance can be taken. The misalignment of the two images may also be due to incorrect diopter adjustment, which can be re-adjusted, or it may be that the magnification of the left and right eyepieces is different. Check eyepieces and reinstall eyepieces of the same magnification. If the image is not clear, it may be that there is dirt on the surface of the objective lens, please clean the objective lens. If the image is not clear when zooming, it may be that the diopter adjustment and focus are not correct, and the diopter adjustment and focus can be re-adjusted. If the bulb often burns out and the light flickers indefinitely, it may be that the local line voltage is too high, the bulb is about to burn out, and the wire connection is short-circuited. Please carefully check whether the voltage and the wire connection of the microscope are secure. It may be that the light bulb is burning out and can be solved by replacing the light bulb. The adjustment of the stereo microscope before use mainly includes: focusing, diopter adjustment, interpupillary distance adjustment and bulb replacement. Each will be described below.


1. Focusing: Put the working table into the mounting hole of the table on the base. When observing transparent specimens, use a frosted glass platform; when observing opaque specimens, use a black and white platform. Then loosen the fastening screw on the focusing slide, and adjust the height of the mirror body to make it roughly the same working distance as the magnification of the selected objective lens. After adjustment, the fastening screws must be tightened. When adjusting the focus, it is recommended to use a flat object, such as a flat paper with printed characters, a ruler, a set square, etc. Diopter adjustment: first adjust the diopter rings on the left and right eyepiece tubes to the 0 reticulated position. Normally, look through the right eyepiece tube first.


Turn the zoom handwheel to the lowest magnification position, turn the focusing handwheel and the diopter adjustment ring to adjust the specimen until the image of the specimen is clear, then turn the zoom handwheel to the highest magnification position and continue to adjust until the image of the specimen is clear At this time, observe with the left eyepiece tube, if it is not clear, adjust the diopter ring on the left eyepiece tube along the axial direction until the image of the specimen is clear.


2. Interpupillary distance adjustment: Pull the two eyepiece tubes to change the exit pupil distance of the two eyepiece tubes.
When the user observes that the two circular fields of view completely overlap, it means that the interpupillary distance has been adjusted. It should be noted that due to the differences in individual vision and eye adjustment, when different users or even the same user use the same stereo microscope at different times, parfocal adjustments should be made separately in order to obtain the best observation Effect. Whether you are replacing the upper light bulb or the lower light bulb, be sure to turn off the power switch and unplug the power cord from the power outlet before replacement.


When replacing the light bulb of the upper light source, first unscrew the knurled screw of the light box of the upper light source, take off the light box, then unload the bad bulb from the lamp holder, replace a good bulb, and then install the light box and the knurled screw. When replacing the light source bulb, it is necessary to take the frosted glass platen or black and white platen out of the base, then remove the bad bulb from the lamp holder and replace it with a good one; then install the frosted glass platen or black and white platen. . When replacing the bulb, please wipe the glass bulb of the bulb with a clean soft cloth or cotton gauze to ensure the lighting effect.

 

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