How to adjust the microscope illumination light path system
In order to make the field of view of the microscope can be subjected to uniform and adequate illumination, in the initial installation and commissioning of the microscope, it is necessary to adjust the illumination optical system, which is an important means of correctly using the microscope and obtaining the correct results and * the basic requirements. In addition, the correct mastery of the adjustment of the illumination light path system is the use of the microscope in the process of replacing the light source bulb after the necessary steps, but also in the daily use of the process from time to time to test the performance of the microscope necessary means. Adjustment of the microscope illumination light path system has the following four main contents:
1, Olympus microscope illumination light source chamber in the microscope outside the initial adjustment
2, ① First of all, open the shell of the lamp chamber, pressure spring clamp halogen bulb into the socket, installation to avoid direct finger contact with the bulb (can be separated by soft cloth or paper), so as not to leave fingerprints and other dirt on the bulb, affecting the service life of the bulb.
3, ② the lamp room on the desktop, turn on the power, use a special screwdriver to adjust the lamp focus knob hole (marked '← →'), so that the filament projection on the wall 1-2m away, the filament imaging adjusted to a clear; and then adjust the lamp high and low position adjusting filament holes (marked '──'), so that the filament imaging to clear; and then adjust the lamp high and low position adjusting filament holes (marked '──'), so that the filament is not in contact with the lamp, so that the filament can be used to adjust the light. '), so that the filament position is appropriate; and then adjust the light of the left and right position of the adjusting screw holes (marked with "──"),so that the filament position is appropriate.
4, the light source luminous body (filament) in the microscope position of the test and correction The purpose is to the luminous body of the image of the end of the end of the objective lens into the field of view of the scope, from the perspective of the light source to ensure that the field of view of the microscope by the full and uniform illumination, which is a prerequisite for adjusting the Kuhler illumination system. Basic tools needed: the centring telescope is already equipped when the microscope is purchased.
① Unplug the hairy glass sleeve from the lamp bank and put the lamp chamber back on the microscope;
② Select the 10 × objective lens, turn on the bright light source procedures to find the sample and focus clear, and then switch to 40 × objective lens to focus the sample clear (40 × objective lens can see the full picture of the filament);
③ Open both the aperture diaphragm and the field of view diaphragm of the spotting scope to * large;
④ Unplug one of the eyepieces, replace it with a centring telescope, grab the white part, and telescope the black receiving eyepiece with the other hand, then you can see the filament image in the field of view;
⑤ If the filament position is not appropriate, adjust the '─' hole, the filament image along the horizontal direction, adjust the '─' hole, the filament image along the vertical direction until the filament image is adjusted to just fill the aperture of the objective lens of the light round image;
⑥ adjustment is complete, the hair glass sleeve inserted back to the original position, pull out the centring telescope, back to the eyepiece for the next step in the adjustment. The adjustment of the illumination light source chamber outside the microscope and the calibration of the position of the light source luminous body inside the microscope mentioned above should only be carried out when the microscope is first installed and debugged and the bulb is replaced, and should not be adjusted randomly when the microscope is used in normal times. In case of disorder, the microscope can be adjusted back to its original state according to the above steps.
Correct Adjustment of Kohler Illumination System One of the main tasks in the correct commissioning of the microscope is the adjustment of the illumination system, and the key to this is the adjustment of the Kohler illumination system. For each person using the microscope, especially for micrographic personnel, should have a certain understanding of the principle of the Kohler illumination system and its adjustment steps and master, in order to give full play to the functions of the microscope should be taken out of the picture in order to effect a more consistent and perfect. The principle of the Kuhler illumination system is simple: the light emitted from any point on the light source luminous body can illuminate the field of view of the microscope, and the light emitted from each point on the light source luminous body to bring together, in the field of view of the microscope to achieve a very full and uniform illumination. The purpose of adjusting the Kühler illumination system is to obtain uniform and sufficient illumination of the observed field of view, to prevent stray light from affecting or interfering with the imaging system, and to avoid the formation of grey haze on the negative during illumination. Necessary components of a highly adjustable Kühler illumination system: a field of view diaphragm, and a condenser mirror system that can be adjusted in conjunction with the axis.
