How to change the microscope's settings for better object visibility
2. Step method:
Turn on the light source
Rotate the coarse adjustment handwheel to separate the objective lens group from the stage to a safe distance
According to experience, change to low power objective lens group and suitable eyepiece group
Place the necessary processed samples on the stage
Adjust eyepiece interpupillary distance
Move the sample through the stage, search the sample and the target area, observe and select the target through the coarse adjustment handwheel
Replace the appropriate high-magnification objective lens and eyepiece, and focus carefully by fine-tuning the handwheel
Research can be carried out as soon as a clear image is obtained, taking pictures if necessary
After finishing the work, turn off the light source and remove the sample. The objective lens group and eyepiece group must also be removed and stored in a dedicated dry and cool place, such as a drying bottle.
2. Matters needing attention:
Focusing skills: When fine-tuning the focus, there must be a process of blurring-clearing-re-blurring. The purpose is to confirm the most accurate focus point found, so you need to turn your head slightly, and then return to the clear focus position.
For samples of certain shapes, color filters can be selected to improve the clarity of details. For example, for substances sensitive to narrow wavelengths and fluorescently stained samples, a color filter of a specific color can be inserted in the optical path. In addition, in order to observe the special organization in the metal sample, the polarizer can be inserted, the angle can be adjusted, and the state of the organization can be studied by observing the specific polarized light reflected by it.
