How to use an inverted Olympus microscope
A well-commissioned Olympus microscope is nothing more than an indication of the basic conditions under which it can be made available for use. However, before each use, depending on the use of different magnification, different optical paths, different imaging systems need to make some adjustments. Before use, first switch on the power. Check whether the number displayed on the voltage dial under the chassis of the main unit is in accordance with the rated input voltage. If the instrument meets the rated voltage, you can turn on the power switch. After the indicator light is on, first give the low voltage to top heat the filament. This will prolong the life of the lamp. Gradually increase the voltage to 6V or 8V, and turn the light conversion marking disc to the BI position. Place the specimen. lox the objective lens into the optical path. Place the spotting scope turntable on the bright field position 'CT'. Adjust the aperture light bar again. When observing specimens with low contrast, make the aperture light bar smaller. It is appropriate to show the contrast of the specimen. Adjust the pupillary distance of the binoculars. Some people's pupil distance between the two eyes is small about 52mm, some people's pupil distance is larger, about 58mm. will be adjusted to the width of the binoculars barrel according to the individual pupil width, food into the field of vision into a field of view into both eyes shall prevail and then adjust the degree of visual acuity, first of all, no visual acuity adjustment to compensate for the eyepieces to adjust the eyepieces to focus. When the specimen to see clearly, and then rotate the other eyepiece of the visual adjustment compensation ring, so that the specimen is equally clear. When taking a micrograph, switch the optical path to the camera to see the crosshairs and frame. Turn the focusing screw to make the crosshairs into a double crosshair, as shown in the figure. The plane of the crosshairs is exactly the same as the plane of the photographic negative.
The imaging system of the simpler inverted Olympus microscope is mounted on an upright single lens barrel with tilting binoculars. On this single barrel, the appropriate magnification of the photographic eyepieces (photo long view) is selected. The camera system is inserted from above. When using this type of camera system, it is important to know that there are two optical switching handles. One of them is located in the head of the mirror. When the handle is pulled out, the entire imaging beam is projected towards the camera system. When the handle is pushed in, the entire imaging beam is projected towards the eyepiece. The **optical switching handle is located at the base of the focusing eyepiece. This cylindrical handle shows only a white circular groove in the push-in position. Only a white ring-shaped groove is visible. In this position the entire imaging beam is projected only into the focusing eyepiece. When the handle is pulled outward to the ** snap position, a blue circular groove is visible. In this position the optical path converter directs 80% of the beam to the camera obscura and 20% to the focusing eyepiece. When pulled outward to the third position, a yellow circular groove appears. In this position the entire beam is directed to the other window on the side. The colour of each buckle is not necessarily the same for the optical path converter of the large Olympus microscopes from various manufacturers in various countries. But as long as you understand a by their own trial, can learn to operate.
After manipulating the focusing screw to adjust the focus on the specimen, you should again adjust the field of view of the light bar, so that the edge of the light bar tangent field of view. Adjust the centre of the light source and screw in the filter. Generally a light blue filter or a light grey filter is used for bright field observation.
We have already mentioned in the light and colour section of the special chapter on microcamera; grey filters are non-selectively absorbing all the spectral lines in the light source, so that it is an average reduction in light intensity.
If the distribution of light intensity in the field of view is not uniform, the appearance of bright spots, the available diffusion filter Olympus CX21 45WF. daylight-type colour film when shooting, in order to increase the colour temperature of the light source to 5400K, you can add an orange filter. When using light-type colour film, green or light blue filters can be added to lower the colour temperature of the light source to 3200K. When observing living cells that are sensitive to thermal radiation, a heat-absorbing filter that absorbs infrared light at wavelengths above 760nm should be added. These filters are transparent or slightly yellowish in appearance. When photographing, the camera can be mounted on the OM light path, i.e., in front of the main unit, or on an upright lens barrel, such as the Olympus CK model. However, it is not advisable to connect the camera directly to the main unit when photographing planktonic cells, especially when making microfilms. This is because the camera's shutter movement generates a spreading wave, which can be transmitted to the planktonic fluid at the moment of filming, seriously affecting the filming effect. In order to avoid this kind of spreading wave, the camera can be mounted on a MTU optical path similar to the IMT-2, and an adapter that cannot transmit spreading waves can be added. Multi-optical path mirrors can also be added and connected to Jane.
