How to use oil immersion objective lenses for optical microscopes
When using an oil immersion objective lens, generally do not use the same height adjustment. The same height focusing is only applicable to the original objective lens of each microscope. It is an extremely advantageous and convenient condition when using low-magnification and high-magnification objectives. However, it is subject to certain restrictions when using oil immersion objectives. Generally speaking, when using oil immersion objectives, When observing specimens (slides) without coverslips, it is safer to use the same height adjustment focus. However, for specimens with coverslips, use caution because the working distance of the oil immersion objective lens is very long. Short, the same height was considered during design and assembly for standard thickness coverslips.
When using an oil immersion objective, only drop cedar oil on the specimen piece. After the observation, cleaning work must be carried out in time. If not done in time, the cedar oil will stick to dust, and the dust particles may wear the lens when wiping. The cedar oil will thicken and dry out when exposed to the air for a long time, making wiping difficult. Very detrimental to the instrument. Wipe carefully and gently. First wipe the front end of the oil immersion objective lens once or twice with dry lens cleaning paper to remove most of the oil, then wipe it twice with lens cleaning paper moistened with xylene, and finally wipe it once with dry lens cleaning paper. The cedar oil on the specimen piece can be removed by the "paper pulling method" (that is, cover the cedar oil with a small piece of lens paper, then drop some xylene on the paper, and pull the paper out while it is wet. Do this three or four times in a row. Clean, generally will not damage uncoverslipped smear specimens) Wipe clean. Lens cleaning paper should also be dust-proof. Generally, before use, cut each page into 8 small pieces and store them in a clean small petri dish. It is economical and convenient to use.
