Instructions and precautions for using microscope oil mirrors and high-power mirrors
1. Mirror retrieval and placement: Open the mirror box and remove the microscope from it. When removing the mirror, hold the mirror arm with your right hand and support the mirror base with your left hand, keeping the mirror body upright to prevent the mirror and eyepiece from slipping and throwing out. Gently place the microscope on the experimental table, usually on the left side, 3-4 centimeters away from the edge of the table, with the mirror arm facing towards your chest for easy observation.
2. Cleaning: Check if the microscope is faulty and clean. If there is dust or dirt on the metal part, it can be wiped with a clean soft cloth. If there is dirt on the lens, it should be wiped with lens wiping paper and never wiped with a handkerchief. If there is glue or dirt, a small amount of xylene can be used to clean it.
3. Aiming: Lift the lens tube 1-2 centimeters away from the stage, rotate the objective lens converter to turn the low magnification lens towards the stage, and align it with the central through hole. Then, adjust the iris aperture to a larger size and observe with your left eye through the eyepiece (do not close your right eye tightly). At the same time, rotate the reflector so that it faces the light source. Generally, a flat mirror is sufficient, but a concave mirror can be used when the light is weak. Reuse a spotlight or rainbow aperture to adjust the intensity of light, making the light in the field of view bright, uniform, and not dazzling. During the process of adjusting light, it is important to understand the different roles of reflectors, spotlights, and rainbow apertures in adjusting light.
4. Specimen installation: Place the slide specimen on the stage, ensuring that the side with the cover glass is facing upwards. Otherwise, when observing with a high-power microscope, the focus cannot be adjusted, and the slide specimen is prone to damage. Then use a slicing clip to clamp the glass slide tightly, rotate the screw of the platform mover, and align the material to be observed with the center of the light hole.
5. Low power microscope observation: When observing any specimen, it is necessary to use a low power microscope first because its field of view is large, making it easy to detect the target and determine the area to be observed.
When observing, first rotate the coarse adjustment wheel clockwise and look at the lens from the left side with both eyes, so that the lens tube descends to a distance of about 5 millimeters from the glass slide. Next, use your left eye to observe downwards from the eyepiece (while opening your right eye to facilitate simultaneous observation and painting), and slowly rotate the coarse adjustment wheel counterclockwise and inward to slowly raise the lens until the object being inspected in the field of view is clear. If the objective lens is too far away from the specimen and the object cannot be seen, it should be checked whether the object being tested is on the optical axis. After adjustment, the lens tube should be lowered again and refocused until the object appears and is clear. Simultaneously adjust the rainbow aperture and spotlight to match.
In order to make the object clearer, a fine-tuning wheel can be used at this time, slightly rotating until the object becomes clearer. But it is important to avoid continuously rotating multiple times to avoid damaging the instrument's accuracy. When the fine adjustment wheel does not move, it indicates that the lifting limit has been reached and cannot be forcefully twisted. It is necessary to readjust the coarse adjustment wheel to slightly widen the distance between the objective lens and the specimen, and then rotate the fine adjustment wheel until the object image is clear.
