Instructions for the use of optical microscopes
(1) During the experiment, the microscope should be placed slightly to the left of the table in front of the seat, and the seat should be about 6-7 cm away from the edge of the table.
(2) Turn on the light source switch and adjust the light intensity to the appropriate size.
(3) Rotate the objective converter so that the low magnification lens is facing the through hole on the stage. First, adjust the lens to a distance of about 1-2cm from the stage, then gaze into the eyepiece with your left eye, and then adjust the height of the condenser to the maximum aperture, allowing light to enter the lens tube through the condenser. At this point, the field of view is bright.
(4) Place the glass slide to be observed on the stage so that the observed part of the slide is located in the center of the through-hole, and then clamp the slide with a specimen clamp.
(5) First, observe with a low magnification lens (objective lens 10X, eyepiece 10x). Before observation, rotate the coarse focusing handwheel to raise the stage and gradually bring the objective lens closer to the glass slide. Please note that the objective lens should not touch the glass slide to prevent it from being crushed by the lens. Then, gaze into the eyepiece with your left eye, while keeping your right eye open (cultivate the habit of using a microscope to observe with your right eye, so that you can use your right eye to look at the drawing while observing), and rotate the coarse focus handwheel to slowly lower the stage. Soon, you can see the magnified image of the material in the glass slide.
(6) If the object seen in the field of view does not meet the experimental requirements (the object deviates from the field of view), the moving handle of the stage can be slowly adjusted. When adjusting, it should be noted that the direction of the slide's movement is exactly opposite to the direction of the object image seen in the field of view. If the object is not very clear, you can adjust the micro focus handwheel until the object is clear.
(7) If using a high-power objective for further observation, the part of the object that needs to be magnified for observation should be moved to the center of the field of view before switching to a high-power objective (when switching from a low-power objective to a high-power objective for observation, the range of the object in the field of view is significantly reduced). Generally, microscopes with normal functions have low magnification and high magnification objectives that are basically in focus. When observing clearly with a low magnification objective, changing to a high magnification objective should show the object image, but the object image may not be very clear. You can adjust it by turning the micro focusing handwheel.
(8) After converting to a high-power objective and seeing the object clearly, the size of the aperture aperture or the height of the condenser can be adjusted as needed to meet the requirements of the light (generally, when switching from a low-power objective to a high-power objective for observation, the field of view needs to be slightly darker, so the intensity of the light needs to be adjusted).
(9) After observation, the objective lens should be moved away from the light hole, and then the aperture aperture should be adjusted to the maximum. Then, the stage should be slowly lowered and the parts should be checked for damage (special attention should be paid to checking whether the objective lens is stained with water or oil. If it is stained with water or oil, it should be wiped clean with lens paper). After inspection and processing, it can be packed.
