Microscope stage micrometer use
Micrometer use:
The micrometer is divided into the eyepiece micrometer and the stage micrometer, and the two rulers are used together. The eyepiece micrometer is a circular piece of glass with a chi in the center, 5~10mm long, divided into 50~100 divisions. The actual length represented by each varies with the magnification of different objectives and with different tube lengths. The stage micrometer is in the center of a glass slide, sealed with gum to seal a round side micrometer, 1~2mm long, divided into 100 or 200 grids. The actual length of each grid is 0.01mm (10μm). When measuring cell size with an eyepiece micrometer, it is necessary to first verify the actual length represented by each division of the eyepiece micrometer with a stage micrometer.
Instructions:
1. Pull out one eyepiece from the lens barrel, unscrew the lower part of the eyepiece, put the eyepiece side micrometer scale down on the focal plane of the eyepiece, reinstall the unscrewed part back into the eyepiece, and then insert the eyepiece back into the lens barrel .
2. Put the micrometer scale on the side of the stage up and clamp it on the stage so that the graduation of the side micrometer is in the center of the field of view. Adjust the focus until the graduations of the micrometer on the side of the stage can be seen clearly.
3. Carefully move the micrometer on the stage side and turn the micrometer on the eyepiece side so that the straight line on the left side of the two feet coincides, and then find another overlapping straight line of the two feet from left to right.
4. Record the grid numbers of the micrometer on the eyepiece side and the micrometer on the stage side between the two coincident lines. Calculate the actual length represented by each division of the micrometer on the side of the eyepiece. The actual length represented by each grid of the eyepiece ruler = (the number of grids of the stage micrometer between the two coincident lines/the number of grids of the eyepiece micrometer between the two coincident lines) × 10um
5. Take off the micrometer on the side of the stage, replace it with the slide specimen to be measured, and measure the specimen with the micrometer on the eyepiece side
