Operating steps and precautions for an upright microscope
1. Placement
Hold the mirror arm with your right hand and the mirror base with your left hand, keeping the mirror body upright. The desktop should be clean and stable, and a window facing or well lit area should be chosen. The single tube is usually placed on the left side, 3-4 centimeters away from the table edge.
2. Cleaning
Check if the microscope is faulty and clean. The mechanical part of the mirror body can be wiped with a clean soft cloth. The lens should be wiped with lens wiping paper. If there is glue or dirt, a small amount of xylene can be used to clean it.
3. Aiming light
Raise the lens tube to a distance of 1-2 centimeters from the stage, and align the low-power lens with the light hole. Adjust the aperture and reflector. When the light is strong, use a flat mirror. When the light is weak, use a concave mirror. The reflector should be rotated with both hands.
If a microscope with a light source is used, the secondary step can be omitted, but a knob for adjusting the brightness is required.
4. Install specimen
Place the glass slide on the stage, ensuring that the side with the cover glass is facing upwards. Fix the glass slide with a spring clip and rotate the knob of the platform shifter to align the material to be observed with the center of the light hole.
5. Focusing
When focusing, first rotate the coarse focusing knob to slowly lower the lens barrel, and carefully observe from the side until the objective lens is close to the glass specimen. Then, observe with the left eye from the eyepiece, and rotate the coarse focusing knob with the left hand to lift the lens barrel until the specimen image is clearly visible. Then, use the fine focusing knob to recall clarity.
Operation note: Do not focus directly under a high-power lens; When the lens tube descends, the distance between the lens tube and the specimen should be observed from the side; To understand the critical value of object distance.
If a binocular microscope is used, if there is a difference in the binocular vision of the observer, the reliable vision adjustment circle should be used for adjustment. In addition, the two cylinders can be relatively translational to adapt to the distance between the operator's eyes.
6. Observation
If using a single tube microscope, open both eyes naturally, observe the specimen with the left eye, record and plot with the right eye, and adjust the focal length with the left hand to make the object clear and move the specimen's field of view. Record and draw with the right hand.
During microscopic examination, the specimen should be moved in a certain direction until the entire specimen is observed, so as not to miss or repeat the examination.
Adjustment of light intensity: In general, the light should be strong for stained specimens, and weak for colorless or unstained specimens; The light should be weak when observing with a low-power lens, and strong when observing with a high-power lens. In addition to adjusting the reflector or light source, the adjustment of the iris aperture is also very important.
(1) Low magnification observation
When observing any specimen, it is necessary to first use a low-power microscope because its field of view is large, making it easy to detect the target and determine the area to be observed.
(2) High magnification observation
When switching from low magnification to high magnification, just slightly adjust the fine focusing knob to make the object image clear.
Do not use a coarse focusing knob when using a high-power lens, as it can easily crush the cover glass and damage the lens.
When rotating the objective lens converter, do not use your fingers to directly push the objective lens. This can easily cause the optical axis of the objective lens to deviate, causing uneven force on the converter threads and damage, ultimately leading to the converter being scrapped.
(3) Observation of oil mirror
First, use a low-power lens and a high-power lens to move the object to the center of the field of view, and then change the oil lens for observation. Before observing with an oil mirror, the brightness of the microscope should be adjusted to * bright and the aperture should be fully opened.
When using an oil lens, first add a drop of cedar oil (lens oil) to the cover glass, then lower the lens tube and carefully observe from the side until the oil lens is immersed in cedar oil and close to the glass specimen. Then observe with an eyepiece and use a fine focusing knob to lift the lens tube until the focal segment of the specimen is clearly visible, and stop and adjust it clearly.
Add an appropriate amount of cedar oil dropwise. After using the oil mirror, it is necessary to wipe off the cedar oil with xylene dipped in lens wiping paper, and then use dry lens wiping paper to wipe off excess xylene.
7. End operation
After observation, remove the sample, twist the converter, and make the V-shaped lens slant to both sides. The reflector should be upright, lower the lens tube, wipe it clean, and put on the lens cover.
If you are using a microscope with a light source, you need to adjust the brightness knob to turn the brightness to * dark, and then turn off the power button to prevent excessive current from burning out the light source during the next startup.
