Polarizing microscope use and precautions
Polarizing microscope is a kind of microscope used to study so-called transparent and opaque anisotropic materials. It has important applications in geology and other science and engineering majors. All substances with birefringence can be clearly distinguished under a polarizing microscope. Of course, these substances can also be observed by staining, but some of them are not available, and a polarizing microscope must be used. The reflective polarizing microscope is an essential instrument for the research and identification of birefringent substances by using the polarization characteristics of light. It can be used for single polarized light observation, orthogonal polarized light observation, and conoscopic light observation.
use
Polarizing microscope is an important instrument for studying the optical properties of crystals, and it is also the basis of other crystal optical research methods (oil immersion method, Freund's method, etc.).
Polarizing microscope is an essential instrument for the research and identification of birefringent substances by using the polarization characteristics of light. It can be used for single polarized light observation, orthogonal polarized light observation, and conoscopic light observation. A method of changing ordinary light into polarized light for microscope inspection to identify whether a substance is single refraction (isotropic) or birefringence (anisotropy).
Birefringence is a fundamental characteristic of crystals. Therefore, polarizing microscopes are widely used in mineral, chemical and other fields. In human and zoology, polarized light microscopy is often used to identify bones, teeth, cholesterol, nerve fibers, tumor cells, striated muscle and hair. Today, I will introduce the application fields of polarizing microscopes.
1. Biological field:
In living organisms, different fibrin structures display pronounced anisotropy, and the use of polarized light microscopy yields details of the molecular arrangement in these fibers. Such as collagen, spinning silk during cell division, etc.
2. Identification of various biological and non-biological materials: such as starch property identification, pharmaceutical composition identification, fiber, liquid crystal, DNA crystal, etc.
3. Medical analysis: such as stones, uric acid crystal detection, arthritis, etc.
4. Geological and mineral analysis:
In addition to common biomedical applications, polarized light microscopes can also be used for polarized light detection of various minerals and crystals, and are widely used in petroleum, mining and semiconductor industries. LED lighting and special filters can be applied in quality control and industrial analysis.
In order to ensure the service life and reliability of the system, pay attention to the following items:
1. The laboratory should have three anti-conditions: shockproof (away from the source of shock), moisture-proof (use air conditioner, dryer), dust-proof (floor on the ground); power supply: 220V+-10%, 50HZ temperature: 0-40 degrees.
2. When adjusting the focus, be careful not to let the objective lens touch the sample, so as not to scratch the objective lens.
3. Do not switch the objective lens when the center of the round hole of the stage spacer is close to the center of the objective lens, so as not to scratch the objective lens.
4. Don't adjust the brightness suddenly, and don't be too bright, which will affect the service life of the bulb and also damage the eyesight.
5. For all (function) switching, the action should be light and in place.
6. Turn off the brightness to the minimum.
7. Non-professionals should not adjust the lighting system (filament position lamp), so as not to affect the image quality.
8. Pay attention to high temperature when replacing the halogen lamp to avoid burns; be careful not to directly touch the glass body of the halogen lamp with your hands.
9. When the machine is not in use, adjust the objective lens to the lowest state through the focusing mechanism.
10. When the machine is turned off and not in use, do not cover the dust cover immediately, and cover it after it cools down. Pay attention to fire prevention.
11. Optical components that are not frequently used are placed in a drying dish.
12. Non-professionals should not try to wipe the objective lens and other optical components. The eyepiece can be wiped with a cotton swab dipped in a 1:1 ratio (absolute alcohol: ether) mixed liquid, and then wiped. Do not use other liquids to avoid damage to the eyepiece.
