Precautions for using a fluorescence microscope
(1) Strictly follow the instructions of the fluorescence microscope factory and do not change the program arbitrarily.
(2) Inspection should be conducted in a dark room. After entering the darkroom, connect the power and light the ultra-high pressure mercury lamp for 5-15 minutes. After the light source emits strong light and stabilizes, the eyes should fully adapt to the darkroom before starting to observe the specimen.
(3) To prevent UV damage to the eyes, protective glasses should be worn when adjusting the light source.
(4) The inspection time should be 1-2 hours each time. If it exceeds 90 minutes, the luminous intensity of the ultra-high pressure mercury lamp gradually decreases and the fluorescence weakens; After 3-5 minutes of exposure to ultraviolet radiation, the fluorescence of the specimen also significantly decreased; So, it should not exceed 2-3 hours at most.
(5) The lifespan of the fluorescent microscope light source is limited, and the specimen should be centrally examined to save time and protect the light source. When the weather is hot, an electric fan should be added to dissipate heat and cool down. When replacing a new light bulb, the usage time should be recorded from the beginning. When the light is turned off and you want to use it again, you must wait for the light bulb to cool sufficiently before igniting it. Avoid lighting the light source several times a day.
(6) Observe the specimen immediately after staining, as the fluorescence will gradually weaken over time. If the specimen is stored in a polyethylene plastic bag at 4 ℃, it can delay the fluorescence attenuation time and prevent the evaporation of the mounting agent.
(7) The judgment criteria for fluorescence brightness are generally divided into four levels, namely "one" - no or visible weak fluorescence. Only visible fluorescence can be seen. Visible with bright fluorescence. Visible and dazzling fluorescence.
