resolution of the Digital microscope
The resolution of the microscope refers to the minimum distance between two object points that can be clearly distinguished by the microscope, also known as the "discrimination rate". Its calculation formula is σ=λ/NA
where σ is the minimum resolution distance; λ is the wavelength of light; NA is the numerical aperture of the objective lens. The resolution of the visible objective lens is determined by the NA value of the objective lens and the wavelength of the illumination light source. The larger the NA value, the shorter the wavelength of the illumination light, the smaller the σ value, and the higher the resolution.
To increase the resolution, i.e. reduce the value of σ, the following measures can be taken
(1) Reduce the wavelength λ value and use a short wavelength light source.
(2) Increase the n value of the medium to increase the NA value (NA=nsinu/2).
(3) Increase the aperture angle u value to increase the NA value.
(4) Increase the contrast between light and dark.
