Standard Operating Procedures for Optical Microscopes
(1) During the experiment, the microscope should be placed slightly to the left on the table in front of it, and the mirror holder should be about 6-7cm away from the edge of the table.
(2) Turn on the light source switch and adjust the light intensity to the appropriate size.
(3) Rotate the objective lens converter to align the low magnification lens with the through-hole on the stage. First, adjust the lens to a distance of about 1-2cm from the stage, then use the left eye to gaze into the eyepiece. Next, adjust the height of the condenser and adjust the aperture stop to large, so that the light passes through the condenser and enters the tube. At this point, the field of view is bright.
(4) Place the glass slide to be observed on the stage so that the observed part of the slide is located in the center of the light hole, and then clamp the slide with the specimen clamp.
(5) First, observe with a low magnification lens (objective 10X, eyepiece 10X). Before observation, turn the coarse focusing handwheel to raise the stage and gradually bring the objective lens closer to the glass slide. Please note that the objective lens should not touch the glass slide to prevent it from crushing the slide. Then, gaze into the eyepiece with your left eye, while keeping your right eye open (develop the habit of opening your eyes and observing with a microscope so that you can use your right eye to look at the drawing while observing), and turn the coarse focusing handwheel to slowly lower the stage. Soon, you will be able to see the magnified image of the material on the slide.
(6) If the object image seen in the field of view does not meet the experimental requirements (the object image deviates from the field of view), the stage moving handle can be slowly adjusted. When adjusting, it should be noted that the direction of movement of the slide is exactly opposite to the direction of movement of the object image seen in the field of view. If the image is not very clear, you can adjust the micro focus handwheel until the image is clear.
(7) If further using a high-power objective for observation, the part of the object image that needs to be magnified for observation should be moved to the center of the field of view before converting to a high-power objective (when converting a low-power objective to a high-power objective for observation, the range of the object image in the field of view is greatly reduced). Generally, microscopes with normal functions have low magnification and high magnification objectives that are basically in focus. When observing clearly with a low magnification objective, changing to a high magnification objective should allow the object image to be seen, but the object image may not be very clear. The micro adjustment focusing handwheel can be turned for adjustment.
(8) After converting to a high magnification objective and seeing the object image clearly, the size of the aperture stop or the height of the condenser can be adjusted as needed to make the light meet the requirements (generally, when switching from a low magnification objective to a high magnification objective for observation, the field of view will slightly darken, so it is necessary to adjust the strength of the light).
(9) After observation, the objective lens should be moved away from the light hole first, then the aperture stop should be adjusted to , and the stage should be slowly lowered to check for any damage to the parts (especially paying attention to checking whether the objective lens is wet or oily, if it is wet or oily, it should be wiped clean with lens paper). After inspection and processing, it can be packed.
