Step-by-step placement using the microscope

Jul 25, 2023

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Step-by-step placement using the microscope

 

Q: The steps to use the microscope are placed
answer:


1. Taking mirror and placing it
1. Hold the mirror arm with your right hand and the mirror base with your left hand.
2. Put the microscope on the experimental table, slightly to the left (the microscope is placed about 7 cm away from the edge of the experimental table
place). Install the eyepiece and objective lens.

Second, the light
3. Turn the converter to align the low magnification objective lens with the light hole (keep a distance of 2 cm between the front end of the objective lens and the stage).
4. Align a larger aperture with the clear aperture. Look into the eyepiece with the left eye (open the right eye for later drawing at the same time). Turn the reflector so that the light is reflected into the lens barrel through the light hole. Through the eyepiece, a bright white field of view can be seen.


3. Observation
5. Put the slide specimen to be observed (it can also be made of a thin paper sheet printed with the word "6") on the stage, and press it with a pressure clamp, and the specimen should be right in the center of the light hole.
6. Rotate the coarse focus screw to lower the lens barrel slowly until the objective lens is close to the slide specimen (the eyes look at the objective lens,
to prevent the objective lens from hitting the slide specimen).
7. Look into the eyepiece with the left eye, and at the same time turn the coarse focus screw in the opposite direction, so that the lens barrel rises slowly until the object is clearly seen as
end. Then turn the fine focus screw slightly to make the object image clearer.
8. Use of high magnification objective lens: Before using high magnification objective lens, you must find the observed object with low magnification objective lens and adjust to
The center of the field of view, then turn the converter and change the high power lens. After switching to a high-power lens, the brightness in the field of view becomes darker, so generally
Choose a larger aperture and use the concave surface of the mirror, then adjust the fine focus screw. fewer objects are viewed, but
The volume becomes larger.
4. Finishing
8. After the experiment, wipe the surface of the microscope clean. Rotate the converter to shift the two objective lenses to the sides and
The barrel slowly descends to the lowest point, and the reflector is placed vertically. Finally put the microscope into the mirror case and send it back to its original place.
"Precautions":
1. It is strictly forbidden to extract the microscope with one hand.
2. If it is necessary to move the microscope, be sure to lift the microscope and put it in a proper position. It is strictly forbidden to push the microscope (the
The resulting vibration may cause loosening of the internal parts of the microscope, remember!!), please handle the microscope with care.
3. When using a microscope, the height of the chair should be appropriate. When observing, you should be used to observing with both eyes at the same time, and the aperture and light source should be bright.
The temperature should be appropriate, otherwise it is easy to feel tired when observing for a long time.
4. Be sure to lower the stage to the lowest point when turning the rotating disk, so as not to scratch the lens of the eyepiece due to improper operation.
5. The slides should be removed for specimen staining or any other operation, and then put back on the stage for observation after the operation is completed.
Operate on the stage to prevent dyes or other liquids from flowing into the microscope or damaging the lens.
6. After observing one material, when you want to change another material, be sure to lower the stage to the lowest point.
Then refocus according to the standard procedure, do not directly replace the specimen, so as not to scratch the lens or slide specimen.
7. After using the microscope, the stage should be lowered to the lowest point, and the low-magnification mirror should be aligned with the central hole of the stage, the power cord should be rolled up, covered with a dust cover, and stored in a storage cabinet

 

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