Steps for Replacing a Low Power Mirror with a High Power Mirror in a Microscope
As is well known, during the use of a microscope, the sample to be magnified and observed is usually first observed with a low-power lens, then moved to the center of the field of view, and finally replaced with a high-power lens for observation. Therefore, there is a transition between low-power and high-power during use, which is commonly known as low-power lens for field of view and high-power lens for observing the sample. This is also the most basic common knowledge used in microscopes, but the methods used are different, The following will provide a detailed introduction to the usage methods between low-power mirrors, high-power mirrors, and oil mirrors.
Steps for using low-power mirrors
When observing any specimen, it is necessary to first use a low-power microscope because its field of view is large, making it easy to detect the target and determine the area to be observed.
(1) Mirror retrieval and placement
Microscopes are high-precision optical experimental instruments that are usually packaged and placed in boxes when not in use. They are then taken out and opened when in use. When taking out the mirror, the right hand tightly grasps the mirror arm and the left hand holds the mirror base to keep the mirror body upright to prevent the mirror and eyepiece from slipping and throwing out. Gently place the microscope on the experimental table, usually on the left side, 3-4 centimeters away from the table edge, with the mirror arm facing towards your chest for easy observation.
(2) Cleaning
Check if the microscope is faulty and clean. If there is dust or dirt on the metal part, it can be wiped with a clean soft cloth. If there is dirt on the lens, it should be wiped with wiping paper, never with a handkerchief. If there is glue or dirt, a small amount of xylene can be used to clean it.
(3) Aiming steps
Raise the lens tube to a distance of 1-2 centimeters from the stage, rotate the objective lens converter, and turn the low magnification lens towards the stage, aligning it with the central light hole. During the rotation process, it should be slow. When you hear a click sound, open the aperture, raise the concentrator, and turn the reflector towards the light source. Observe with your left eye on the eyepiece (do not close your right eye tightly), while adjusting the direction of the reflector until the light in your field of view is evenly bright.
(4) Placing slide specimens
Take a glass slide specimen and place it on the stage, making sure that the side with the cover glass is facing upwards. Otherwise, when observing with a high-power microscope, the focus cannot be adjusted, and the glass slide specimen is prone to damage. Then use a slicing clip to clamp the glass slide tightly, rotate the screw of the platform shifter, and align the area to be observed with the center of the light hole.
(5) Adjusting the focal length
Rotate the coarse adjuster counterclockwise with your left hand to slowly raise the lens to a distance of about 5 millimeters from the objective lens to the specimen. Be careful not to observe on the eyepiece when raising the lens. Be sure to watch the mirror stand rise from the right side to avoid excessive rise and damage to the lens or specimen. Then, open both eyes at the same time, observe with your left eye on the eyepiece, and slowly rotate the coarse adjuster clockwise with your left hand to slowly lower the lens until a clear object appears in your field of view.
Note: If the object image is not in the center of the field of view, the slide pusher can be adjusted to center it (note that the direction of moving the slide is opposite to the direction of moving the object image in the field of view). If the brightness in the field of view is not suitable, it can be adjusted by adjusting the position of the concentrator or the size of the opening and closing aperture. If the lens table drops beyond the working distance (>5.40mm) and no object is seen during the adjustment of the focal length, it indicates that the operation has failed. Therefore, the operation should be repeated and must not be repeated
