Steps for using an optical microscope
Operating Steps and Precautions
（1） Upright microscope
1. Place your right hand to hold the mirror arm and your left hand to support the mirror base, keeping the mirror body upright. The desktop should be clean and stable, and a window facing or well lit area should be chosen. The single tube is usually placed on the left side, 3-4 centimeters away from the table edge.
2. Clean and inspect the microscope for any defects and cleanliness. The mechanical part of the mirror body can be wiped with a clean soft cloth. The lens should be wiped with lens wiping paper. If there is glue or dirt, a small amount of xylene can be used to clean it.
3. Raise the aiming lens tube to a distance of 1-2 centimeters from the stage, and align the low-power lens with the light hole. Adjust the aperture and reflector. When the light is strong, use a flat mirror. When the light is weak, use a concave mirror. The reflector should be rotated with both hands. If a microscope with a light source is used, the secondary step can be omitted, but a knob for adjusting the brightness is required.
4. Install the specimen and place the slide on the stage, ensuring that the side with the cover slide is facing upwards. Fix the glass slide with a spring clip and rotate the knob of the platform shifter to align the material to be observed with the center of the light hole.
5. When focusing, first rotate the coarse focusing knob to slowly lower the lens barrel, and carefully observe from the side until the objective lens is close to the glass specimen. Then, observe with the left eye from the eyepiece, and rotate the coarse focusing knob with the left hand to lift the lens barrel until the specimen image is clearly visible. Then, use the fine focusing knob to adjust back to clarity. Operation note: Do not focus directly under a high-power lens; When the lens tube descends, the distance between the lens tube and the specimen should be observed from the side; To understand the critical value of object distance. If a binocular microscope is used, if there is a difference in the binocular vision of the observer, the reliable vision adjustment circle should be used for adjustment. In addition, the two cylinders can be relatively translational to adapt to the distance between the operator's eyes.
6. If a single tube microscope is used for observation, the two eyes should naturally open, the left eye should observe the specimen, the right eye should observe the recording and drawing, and the left hand should adjust the focal length to make the object clear and move the specimen's field of view. Record and draw with the right hand. During microscopic examination, the specimen should be moved in a certain direction until the entire specimen is observed, so as not to miss or repeat the examination. Adjustment of light intensity: In general, the light should be strong for stained specimens, and weak for colorless or unstained specimens; The light should be weak when observing with a low-power lens, and strong when observing with a high-power lens. In addition to adjusting the reflector or light source, the adjustment of the iris aperture is also very important.
1) When observing any specimen under a low-power microscope, it is necessary to first use a low-power microscope because its field of view is large, making it easy to detect the target and determine the area to be observed.
2) When observing with a high-power lens from a low-power lens to a high-power lens, just slightly adjust the fine focusing knob to make the object image clear. Do not use a coarse focusing knob when using a high-power lens, as it can easily crush the cover glass and damage the lens. When rotating the objective lens converter, it is not advisable to directly push the objective lens with your fingers. This can easily cause the optical axis of the objective lens to deviate, causing uneven force on the converter threads and damage, resulting in the converter being scrapped.
(3) To observe with an oil mirror, first use a low-power or high-power lens to move the object to the center of the field of view, and then change the oil mirror for observation. Before observing with an oil mirror, the brightness of the microscope should be adjusted to * bright and the aperture should be fully opened. When using an oil lens, first add a drop of cedar oil (lens oil) to the cover glass, then lower the lens tube and carefully observe from the side until the oil lens is immersed in cedar oil and close to the glass specimen. Then observe with an eyepiece and use a fine focusing knob to lift the lens tube until the focal segment of the specimen is clearly visible, and stop and adjust it clearly. Add an appropriate amount of cedar oil dropwise. After using the oil mirror, it is necessary to wipe off the cedar oil with xylene dipped in lens wiping paper, and then use dry lens wiping paper to wipe off excess xylene.
