The Function of Filters in Microscopic Inspection of Leica Stereo MicroscopesThe Function of Filters in Microscopic Inspection of Leica Stereo Microscopes
The filter is also called the color filter, and its role in the microscopic inspection and photomicrograph of the Leica stereo microscope cannot be ignored. Reasonable selection of filters can improve image contrast, resolution and contrast enhancement; in color microphotography, it can adjust the color temperature of the light source.
Object points, primary and complementary colors for the visible spectrum
We know that the hue of each light element in the microscope inspection of a Leica stereo microscope is related to their respective wavelengths. When ordinary light passes through the spectroscope, it is divided into a continuous spectrum of red, orange, yellow, green, cyan, blue, and purple. This spectrum is composed of three primary colors - blue, green and red, which are continuous with each other through color changes:
The combination of the three primary colors can produce white light under proper conditions; the combination of the other two primary colors can produce other colors.
If the light reflected by a colored object or the light transmitted by colored glass is analyzed in the spectroscope, it will be found that part of the above-mentioned continuous spectrum is missing or part of it is missing. The missing portion appears as a dark band, called the "transmitted light band".
In the microscope inspection of Leica stereo microscope, in addition to complementary colors that can absorb each other, putting a red filter in the visible spectrum can absorb blue and green colors and pass through the red light band with a wavelength of 600-700nm; a green filter can absorb blue and red colors and pass through the green part with a wavelength of 500-600nm; a blue filter absorbs red and green colors and pass through the blue part with a wavelength of 400-500nm.
It can be seen from this that a certain color filter can pass a certain color light and absorb it, but there is a difference in the amount of absorption of its color light. For example, the green filter cannot completely absorb the blue and red dichroic light, and not all the green light can pass through, but the green light passes through much more than the blue and red dichromatic light. In the same way, although the red filter can pass most of the red light, it also has a small amount of orange light and weak green, blue and purple light. In addition, the darker the color of the filter, the stronger the absorption performance.
The filter is also called the color filter, and its role in the microscopic inspection and photomicrograph of the Leica stereo microscope cannot be ignored. Reasonable selection of filters can improve image contrast, resolution and contrast enhancement; in color microphotography, it can adjust the color temperature of the light source.
Object points, primary and complementary colors for the visible spectrum
We know that the hue of each light element in the microscope inspection of a Leica stereo microscope is related to their respective wavelengths. When ordinary light passes through the spectroscope, it is divided into a continuous spectrum of red, orange, yellow, green, cyan, blue, and purple. This spectrum is composed of three primary colors - blue, green and red, which are continuous with each other through color changes:
The combination of the three primary colors can produce white light under proper conditions; the combination of the other two primary colors can produce other colors.
If the light reflected by a colored object or the light transmitted by colored glass is analyzed in the spectroscope, it will be found that part of the above-mentioned continuous spectrum is missing or part of it is missing. The missing portion appears as a dark band, called the "transmitted light band".
In the microscope inspection of Leica stereo microscope, in addition to complementary colors that can absorb each other, putting a red filter in the visible spectrum can absorb blue and green colors and pass through the red light band with a wavelength of 600-700nm; a green filter can absorb blue and red colors and pass through the green part with a wavelength of 500-600nm; a blue filter absorbs red and green colors and pass through the blue part with a wavelength of 400-500nm.
It can be seen from this that a certain color filter can pass a certain color light and absorb it, but there is a difference in the amount of absorption of its color light. For example, the green filter cannot completely absorb the blue and red dichroic light, and not all the green light can pass through, but the green light passes through much more than the blue and red dichromatic light. In the same way, although the red filter can pass most of the red light, it also has a small amount of orange light and weak green, blue and purple light. In addition, the darker the color of the filter, the stronger the absorption performance.
