The metallographic microscope adjusts the aperture, condenser and brightness to uniformly illuminate the field of view.
1. Appropriately adjust the aperture, condenser and brightness to ensure uniform illumination of the field of view.
2. First use a low-magnification lens, that is, a 10x objective lens, to observe. Raise the stage to a distance of approximately 5mm between the slide and the bottom of the objective lens. Then slowly lower the stage with the coarse adjustment knob while observing through the eyepiece. until an image appears in the field of view. This prevents damage to the objective lens from contact with the slide during observation. When an image appears in your field of view, use the fine adjustment screw to adjust the image until it is clear. Note: The fine adjustment knob cannot be over-adjusted in the same direction.
3. Switch to the high power lens, that is, the 40x objective lens. At this time, the specimen should still be in the field of view, but it will be 4 times larger than before. When using a parfocal objective, use the fine adjustment knob to adjust the object image until it is clear. If a non-parfocal objective is used, the focus must be re-adjusted each time the objective is changed. When switching from low magnification to high magnification, first raise the stage to a position very close to the slide, and then follow step 4 to adjust again. Fungal specimens can be clearly observed with a high-power microscope.
5. Use an oil lens to observe stained bacterial specimens: lower the stage, add a drop of cedar oil to the position on the slide where the light source passes through, then turn the 100x objective lens, the oil lens, into the light path, and use the coarse adjustment knob to raise the load. Place the object on the stage and view from the side so that the lens is immersed in the oil and almost in contact with the slide. Note: Do not use excessive force to avoid crushing the glass and damaging the lens.
6. Observe through the eyepiece and increase the brightness to maximum. Use the coarse adjustment knob to slowly lower the stage until the object image appears, then use the fine adjustment knob to adjust until the object image is clear.
7. When using an oil lens, once the lens comes into contact with cedar oil, do not turn the high-power lens in again. Otherwise, the high-magnification lens will need to be cleaned thoroughly due to oil stains.
8. When the observation is completed, use lens cleaning paper to wipe off the remaining cedar oil on the oil lens. You can also use xylene to perform this step, but you must also use lens cleaning paper to wipe off the remaining xylene. Microscope lenses should only be cleaned with lens tissue when necessary.
9. Turn the 4x objective lens in and lower the stage.
10. Return the microscope to its storage location.
