The procedure for using a regular optical microscope
1. Preparation before microscopy
The room should be clean and dry, the benchtop should be level, stable and free of vibration, and no corrosive reagents should be placed near the microscope. When taking out the microscope from the microscope cabinet or mirror box, hold the mirror arm tightly with your right hand, hold the mirror base with your left hand, take it out smoothly, place it on the tabletop of the test bench, and place it in front of the left of the operator, about 10cm away from the edge of the test bench. Arms facing you, barrel facing forward. The drawing utensils are placed on the right side of the laboratory table.
2. adjust the light source
If you need to use an external light source, diffused natural light or soft light should be used. Direct sunlight can damage the observer's eyes. Turn the converter so that the low-power mirror is facing the light hole, open the iridescent aperture on the condenser to the maximum, observe the brightness of the field of view in the eyepiece, and adjust the angle of the mirror at the same time to make the light the brightest and most uniform. The microscope with its own light source can adjust the intensity of light by adjusting the current knob.
3. Device to be inspected slide
The sample to be observed is made into a temporary or permanent mount, placed on the stage, fixed with a spring clip, and the side with the cover glass facing up. Move the pusher and adjust the sample to be tested to the center of the light hole.
4. Observation with low magnification
Align the low-magnification lens with the light hole, slowly turn the coarse quasi-focus screw, and adjust the distance between the objective lens and the loading film to the shortest. Be careful not to crush the coverslip. Observe through the eyepiece, and adjust slowly with the coarse focus screw until the object image appears, and then fine-tune with the fine focus screw, and adjust the brightness of the light source and the size of the iridescent aperture at the same time, so that the object image can reach the clearest level. And use the propeller to move the part that needs to be further enlarged and observed to the center of the field of view. If binocular eyepieces are used, the distance between the binoculars should be adjusted before observation so that the visual fields of the two eyes can be merged.
5. High power lens observation
Turn the converter to select a higher magnification objective lens, and use the fine focus screw to adjust the focal length until the object image is clear.
6. oil immersion observation
The working distance of the oil immersion objective lens (refers to the distance between the surface of the front lens of the objective lens and the object under inspection) is very short, generally within 0.2 mm, and the oil immersion objective lens of general optical microscopes does not have a "spring device", so the oil immersion objective lens is used When , the focusing speed must be slowed down to avoid crushing the slide and damaging the objective lens.
(1) Find the observation target under low magnification, gradually zoom in under medium and high magnification, place the part to be observed in the center of the field of vision, adjust the light source and iridescent aperture to maximize the light passing through the condenser.
(2) Turn the coarse quasi-focus screw, rotate the lens barrel up (or lower the stage) about 2 cm, and add a small drop of cedar oil to the microscope inspection part of the slide.
(3) Slowly turn the coarse focus screw back, and observe from the side at the same time, until the oil lens is immersed in the oil drop, and the lens is almost in contact with the specimen.
(4) Observe from the eyepiece and fine-tune with the fine-focus screw until the object image is clear.
(5) After the microscope inspection, rotate the lens away from the glass slide and clean the lens immediately. Generally, first wipe off the cedar oil drops on the lens with a lens tissue. Then dip a little ether alcohol mixture (2:3) with lens tissue to wipe off the residual oil, and finally wipe it off with clean lens tissue (pay attention to wipe in one direction).
7. Reduction microscope
Turn off the built-in light source and unplug the power plug, or make the reflector perpendicular to the condenser. Rotate the revolving nosepiece so that the objective lens is in a figure-eight position facing the light aperture. Then adjust the distance between the lens barrel and the stage to the nearest, and lower the condenser. Put the dust cover on and return the microscope to the cabinet or mirror box.
